Fast atom bombardment

Fast atom bombardment

Infobox chemical analysis
name = Fast atom bombardment mass spectrometry


caption =ThermoQuest AvantGarde MS with quadropule detector and FAB/EI source.
acronym = FAB
classification =Mass spectrometry
analytes =
manufacturers =
related = Secondary ion mass spectrometry
Matrix-assisted laser desorption/ionization
Plasma desorption mass spectrometry
hyphenated =

Fast atom bombardment (FAB) is an ionization technique used in mass spectrometry.cite journal | author = Morris HR, Panico M, Barber M, Bordoli RS, Sedgwick RD, Tyler A | title = Fast atom bombardment: a new mass spectrometric method for peptide sequence analysis | journal = Biochem. Biophys. Res. Commun. | volume = 101 | issue = 2 | pages = 623–31 | year = 1981 | pmid = 7306100 | doi = ] [Barber, M.; Bordoli, R.S.; Sedgewick, R.D.; Tyler, A.N., Nature, 293, 1981, pp270-275] [Barber, M.; Bordoli, R.S.; Elliott, G.J.; Sedgewick, R.D.; Tyler, A.N., Analytical Chemistry, 54, 1982, pp645A-657A.] [cite journal | author=Barber M, Bordoli RS, Sedgewick RD Tyler AN | title=Fast atom bombardment of solids (F.A.B.): a new ion source for mass spectrometry| journal=Journal of the Chemical Society, Chemical Communications | | pages=325–7 year=1981 | doi=10.1039/C39810000325 | year=1981 ] The material to be analyzed is mixed with a non-volatile chemical protection environment called a matrix and is bombarded under vacuum with a high energy (4000 to 10,000 electron volts) beam of atoms. The atoms are typically from an inert gas such as argon or xenon. Common matrices include glycerol, thioglycerol, 3-nitrobenzyl alcohol (3-NBA), 18-Crown-6 ether, 2-nitrophenyloctyl ether, sulfolane, diethanolamine, and triethanolamine. This technique is similar to secondary ion mass spectrometry and plasma desorption mass spectrometry.

How it works

FAB is a relatively soft ionization technique and produces primarily intact protonated molecules denoted as [M+H] + and deprotonated molecules such as [M-H] -. The nature of its ionization products places it close to electrospray and MALDI. [cite journal | author=Tomer KB | title=The development of fast atom bombardment combined with tandem mass spectrometry for the determination of biomolecules| journal=Mass Spectrometry Reviews | volume=8 | pages=445–82 year=1989 | doi=10.1002/mas.1280080602 | year=1989 ]

The first example of the practical application of this technique was the elucidation of the amino acid sequence of the oligopeptide efrapeptin D. This contained a variety of very unusual amino acid residues. [Bullough,D.A., Jackson C.G.,Henderson, P.J.F., Cottee, F.H.,Beechey,R.B. and Linnett, P.E. Biochemistry International (1981) 4, 543-549] The sequence was shown to be: N-acetyl-L-pip-AIB-L-pip-AIB-AIB-L-leu-beta-ala-gly-AIB-AIB-L-pip-AIB-gly-L-leu-L-iva-AIB-X. PIP = pipecolic acid, AIB = alpha-amino-isobutyric acid,leu = leucine, iva = isovaline, gly = glycine. This is a potent inhibitor of the mitochodrial ATPase activity.

References

Bibliography

*cite book |author=Caprioli, R. M. |title=Continuous-flow fast atom bombardment mass spectrometry |publisher=Wiley |location=New York |year=1990 |pages= |isbn=0-471-92863-1 |oclc= |doi=

ee also

* Matrix-assisted laser desorption/ionization
* Soft laser desorption

External links

* [http://www.chemistry.unimelb.edu.au/school/services/MassSpec/homepage/fab.html A good intro to FAB]


Wikimedia Foundation. 2010.

Игры ⚽ Нужна курсовая?

Look at other dictionaries:

  • Fast Atom Bombardment — (FAB) ist ein Ionisierungsverfahren, das in Massenspektrometern (MS) benutzt wird. Dabei wird eine Lösung des Analyts in einer flüssigen schwer flüchtigen Matrix mit einem Partikelstrom aus gewöhnlich inerten Gasen wie Argon oder Xenon… …   Deutsch Wikipedia

  • atom — /at euhm/, n. 1. Physics. a. the smallest component of an element having the chemical properties of the element, consisting of a nucleus containing combinations of neutrons and protons and one or more electrons bound to the nucleus by electrical… …   Universalium

  • Energetic neutral atom — ENAs redirects here. For other uses, see Enas (disambiguation). Neutral Atom Imaging redirects here. For other uses, see Atom (disambiguation). ENA images of the fluctuation of Earth s ring current during July 15–16, 2000 geomagnetic storm made… …   Wikipedia

  • FAB — Fast Atom Bombardment (Academic & Science » Electronics) *** French American British (Regional) ** Food And Beverage (Community) * Feline Advisory Bureau (Medical » Veterinary) * Fragment Antigen Binding (Medical » Physiology) * Food And Booze… …   Abbreviations dictionary

  • FAB-MS — fast atom bombardment mass spectrometry …   Medical dictionary

  • FAB — • fast atom bombardment; • formalin ammonium bromide; • fragment, antigen binding [of immunoglobulins]; • French American British [leukemia staging]; • functional arm brace …   Dictionary of medical acronyms & abbreviations

  • FAB-MS — • fast atom bombardment mass spectrometry …   Dictionary of medical acronyms & abbreviations

  • Десорбционные методы ионизации в масс-спектрометрии — Десорбционные методы ионизации в масс спектрометрии  группа методов ионизации в масс спектрометрии, для которых процессы десорбции твердого анализируемого вещества и его ионизации практически неотделимы во времени. Наиболее полное развитие… …   Википедия

  • Ion source — An ion source is an electro magnetic device that is used to create charged particles. These are used primarily within mass spectrometers or particle accelerators.Mass spectrometry In mass spectrometry, an ion source is a piece of equipment used… …   Wikipedia

  • Mass spectrometry — (MS) is an analytical technique that measures the mass to charge ratio of charged particles.[1] It is used for determining masses of particles, for determining the elemental composition of a sample or molecule, and for elucidating the chemical… …   Wikipedia

Share the article and excerpts

Direct link
… Do a right-click on the link above
and select “Copy Link”