- Fast atom bombardment
Infobox chemical analysis
name = Fast atom bombardment mass spectrometry
caption =ThermoQuest AvantGarde MS with quadropule detector and FAB/EI source.
acronym = FAB
Secondary ion mass spectrometry Matrix-assisted laser desorption/ionization Plasma desorption mass spectrometry
Fast atom bombardment (FAB) is an
ionizationtechnique used in mass spectrometry.cite journal | author = Morris HR, Panico M, Barber M, Bordoli RS, Sedgwick RD, Tyler A | title = Fast atom bombardment: a new mass spectrometric method for peptide sequence analysis | journal = Biochem. Biophys. Res. Commun. | volume = 101 | issue = 2 | pages = 623–31 | year = 1981 | pmid = 7306100 | doi = ] [Barber, M.; Bordoli, R.S.; Sedgewick, R.D.; Tyler, A.N., Nature, 293, 1981, pp270-275] [Barber, M.; Bordoli, R.S.; Elliott, G.J.; Sedgewick, R.D.; Tyler, A.N., Analytical Chemistry, 54, 1982, pp645A-657A.] [cite journal | author=Barber M, Bordoli RS, Sedgewick RD Tyler AN | title=Fast atom bombardment of solids (F.A.B.): a new ion source for mass spectrometry| journal= Journal of the Chemical Society, Chemical Communications | | pages=325–7 year=1981 | doi=10.1039/C39810000325 | year=1981 ] The material to be analyzed is mixed with a non-volatile chemical protection environment called a matrix and is bombarded under vacuum with a high energy (4000 to 10,000 electron volts) beam of atoms. The atoms are typically from an inert gas such as argonor xenon. Common matrices include glycerol, thioglycerol, 3-nitrobenzyl alcohol(3-NBA), 18-Crown-6ether, 2-nitrophenyloctyl ether, sulfolane, diethanolamine, and triethanolamine. This technique is similar to secondary ion mass spectrometryand plasma desorption mass spectrometry.
How it works
FAB is a relatively soft ionization technique and produces primarily intact protonated molecules denoted as [M+H] + and deprotonated molecules such as [M-H] -. The nature of its ionization products places it close to
electrosprayand MALDI. [cite journal | author=Tomer KB | title=The development of fast atom bombardment combined with tandem mass spectrometry for the determination of biomolecules| journal= Mass Spectrometry Reviews| volume=8 | pages=445–82 year=1989 | doi=10.1002/mas.1280080602 | year=1989 ]
The first example of the practical application of this technique was the elucidation of the amino acid sequence of the oligopeptide efrapeptin D. This contained a variety of very unusual amino acid residues. [Bullough,D.A., Jackson C.G.,Henderson, P.J.F., Cottee, F.H.,Beechey,R.B. and Linnett, P.E. Biochemistry International (1981) 4, 543-549] The sequence was shown to be: N-acetyl-L-pip-AIB-L-pip-AIB-AIB-L-leu-beta-ala-gly-AIB-AIB-L-pip-AIB-gly-L-leu-L-iva-AIB-X. PIP = pipecolic acid, AIB = alpha-amino-isobutyric acid,leu = leucine, iva = isovaline, gly = glycine. This is a potent inhibitor of the mitochodrial ATPase activity.
*cite book |author=Caprioli, R. M. |title=Continuous-flow fast atom bombardment mass spectrometry |publisher=Wiley |location=New York |year=1990 |pages= |isbn=0-471-92863-1 |oclc= |doi=
Matrix-assisted laser desorption/ionization
Soft laser desorption
* [http://www.chemistry.unimelb.edu.au/school/services/MassSpec/homepage/fab.html A good intro to FAB]
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