Diphthine—ammonia ligase

Diphthine—ammonia ligase
diphthine-ammonia ligase
Identifiers
EC number 6.3.2.22
CAS number 114514-33-9
Databases
IntEnz IntEnz view
BRENDA BRENDA entry
ExPASy NiceZyme view
KEGG KEGG entry
MetaCyc metabolic pathway
PRIAM profile
PDB structures RCSB PDB PDBe PDBsum
Gene Ontology AmiGO / EGO

In enzymology, a diphthine-ammonia ligase (EC 6.3.2.22) is an enzyme that catalyzes the chemical reaction

ATP + diphthine + NH3 \rightleftharpoons ADP + phosphate + diphthamide

The 3 substrates of this enzyme are ATP, diphthine, and NH3, whereas its 3 products are ADP, phosphate, and diphthamide.

This enzyme belongs to the family of ligases, specifically those forming carbon-nitrogen bonds as acid-D-amino-acid ligases (peptide synthases). The systematic name of this enzyme class is diphthine:ammonia ligase (ADP-forming). Other names in common use include diphthamide synthase, and diphthamide synthetase.

References

  • Moehring JM, Moehring TJ (1988). "The post-translational trimethylation of diphthamide studied in vitro". J. Biol. Chem. 263 (8): 3840–4. PMID 3346227. 
  • Moehring TJ and Moehring JM (1987). "Mutant cultured cells used to study the synthesis of diphthamide". UCLA Symp. Mol. Cell. Biol. New Ser. 45: 53–63. 
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