Equilibrium gradient centrifugation
- Equilibrium gradient centrifugation
Also called: isopycnic density gradient centrifugation, is a type of centrifugation procedure widely used in biochemistry to separate molecules based on their isopycnic point (Buoyant Density). By spinning biological (or other) preparations at high G-Force over long periods of time, in buffers or solutions containing a varying amount of a viscous molecule (e.g. 0.8 Molar/1.2M Molar sucrose step-gradient used in post-synaptic density isolation or a 20-50% linear Sucrose gradient used in the purification of clathrin coated vesicles (CCVs).
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Sucrose gradient centrifugation — is a type of centrifugation often used to purify enveloped viruses (with densities 1.1 1.2 g/cm³) and ribosomes, and also to separate cell organelles from crude cellular extracts. This method is also used to purify exosomes. [cite journal |… … Wikipedia
density gradient centrifugation — noun (biology) The separation of cells, cell components or complex molecules on the basis of their density differences by centrifuging them to their density equilibrium positions in gradients established in appropriate solutions • • • Main Entry … Useful english dictionary
cesium chloride equilibrium density gradient centrifugation — cesium chloride equilibrium density gradient centrifugation. См. центрифугирование в градиенте плотности хлорида цезия. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995… … Молекулярная биология и генетика. Толковый словарь.
sucrose equilibrium density gradient centrifugation — sucrose equilibrium density gradient centrifugation. См. центрифугирование в градиенте плотности сахарозы. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) … Молекулярная биология и генетика. Толковый словарь.
equilibrium density gradient centrifugation — A procedure used to separate macromolecules based on their density (mass per unit volume) … Glossary of Biotechnology
Centrifugation — is a process that involves the use of the centrifugal force for the sedimentation of mixtures with a centrifuge, used in industry and in laboratory settings. More dense components of the mixture migrate away from the axis of the centrifuge, while … Wikipedia
centrifugation — Subjection to sedimentation, by means of a centrifuge, of solids suspended in a fluid. SYN: centrifugalization. band c. SYN: density gradient c.. density gradient c. ultracentrifugation of substances in concentrated solutions of … Medical dictionary
gradient — Rate of change of temperature, pressure, magnetic field, or other variable as a function of distance, time, etc. atrioventricular g. the diastolic … Medical dictionary
Isopycnic centrifugation — Isopycnic centrifugation, also known as density gradient centrifugation or equilibrium sedimentation is a technique used to separate molecules on the basis of buoyant density. (The word isopycnic means equal density .) Typically, a self… … Wikipedia
Differential centrifugation — is a common procedure in microbiology and cytology used to separate certain organelles from whole cells for further analysis of specific parts of cells. In the process, a tissue sample is first homogenised to break the cell membranes and mix up… … Wikipedia
