URA3

URA3

URA3 encodes orotidine 5-phosphate decarboxylase (ODCase), an enzyme involved in the synthesis of pyrimidine ribonucleotides. Loss of ODCase activity leads to a lack of cell growth unless uracil or uridine is added to the media (positive selection). In contrast, if 5-FOA is added to the media ODCase can convert into the toxic compound 5-fluorouracil causing death (negative selection).

Since URA3 allows for both positive and negative selection, it has been developed as a genetic marker for DNA transformations and other genetic techniques in bacteria and many fungal species. It is one of the most important genetic markers in yeast genetic modification.

References

S. cerevisiae database (2005) URA3 gene. http://db.yeastgenome.org/cgi-bin/locus.pl?locus=ura3#summaryParagraph (accessed 16/10/07).

PAUL J. FLYNN AND RICHARD J. REECE. Activation of Transcription by Metabolic Intermediates of the Pyrimidine Biosynthetic Pathway. MOLECULAR AND CELLULAR BIOLOGY,Vol. 19, No. 1, 882–888


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