Folin's reagent

Folin's reagent

Folin's reagent is 1,2-naphthoquinone-4-sulfonate and is a chemical reagent used to measure levels of amines and amino acids. [cite journal |author=Saurina J, Hernández-Cassou S |title=Determination of amino acids by ion-pair liquid chromatography with post-column derivatization using 1,2-naphthoquinone-4-sulfonate |journal=Journal of chromatography. A |volume=676 |issue=2 |pages=311–9 |year=1994 |pmid=7921184 |doi=10.1016/0021-9673(94)80431-1] The reagent produces a bright red color in alkaline solutions and is also fluorescent. [cite journal |author=Kobayashi Y, Kubo H, Kinoshita T |title=Fluorometric determination of guanidino compounds by new postcolumn derivatization system using reversed-phase ion-pair high-performance liquid chromatography |journal=Anal. Biochem. |volume=160 |issue=2 |pages=392–8 |year=1987 |pmid=3578768 |doi=10.1016/0003-2697(87)90066-2]

This should not be confused with Folin-Ciocalteu reagent, which is a mixture of sodium tungstate and sodium molybdate that is used to detect phenolic compounds.

References

External links

* [http://www.sigmaaldrich.com/catalog/search/ProductDetail/SIGMA/N1766 Sodium 1,2-naphthoquinone-4-sulfonate] Sigma-Aldrich datasheet.
* [http://pubchem.ncbi.nlm.nih.gov/summary/summary.cgi?sid=24886075 PubChem database]


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