Small nucleolar RNA SNORD45

snoRNA U45 (also known as SNORD45) is a non-coding RNA (ncRNA) molecule which functions in the modification of other small nuclear RNAs (snRNAs). This type of modifiying RNA is usually located in the nucleolus of the eukaryotic cell which is a major site of snRNA biogenesis. It is known as a small nucleolar RNA (snoRNA) and also often referred to as a guide RNA.

snoRNA U45 belongs to the C/D box class of snoRNAs which contain the conserved sequence motifs known as the C box (UGAUGA) and the D box (CUGA). Most of the members of the box C/D family function in directing site-specific 2'-O-methylation of substrate RNAs [cite journal | last = Galardi | first = S | coauthors = Fatica A, Bachi A, Scaloni A, Presutti C, Bozzoni I | year = 2002 | title = Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro | journal = Mol Cell Biol | volume = 22 | pages = 6663–6668 | pmid = 12215523 | doi = 10.1128/MCB.22.19.6663-6668.2002] .

U45 was originally cloned from HeLa cells and expression verified by northern blotting cite journal | author = Kiss-László Z, Henry Y, Bachellerie JP, Caizergues-Ferrer M, Kiss T | title = Site-specific ribose methylation of preribosomal RNA: a novel function for small nucleolar RNAs | journal = Cell | volume = 85 | issue = 7 | pages = 1077–88 | year = 1996 | pmid = 8674114 | doi = | issn = ] . It is related to the snoRNA (MBII-401) identified in mouse cite journal | last = Huttenhofer | first = A | coauthors = Kiefmann M, Meier-Ewert S, O'Brien J, Lehrach H, Bachellerie JP, Brosius J | year = 2001 | title = RNomics: an experimental approach that identifies 201 candidates for novel, small, non-messenger RNAs in mouse | journal = EMBO J. | volume = 20 | pages = 2943–2953 | pmid = 11387227 | doi = 10.1093/emboj/20.11.2943 ]

In the human genome there are three very closely related copies of snoRNA U45 (called U45A, U45B, U45C) located within the introns of the same host genecite journal | last = Lestrade | first = L | coauthors = Weber MJ | year = 2006 | title = snoRNA-LBME-db, a comprehensive database of human H/ACA and C/D box snoRNAs | journal = Nucleic Acids Res. | volume = 34 | pages = D158–D162 | pmid = 16381836 | doi = 10.1093/nar/gkj002 ] .

References

External links

*
* [http://www-snorna.biotoul.fr/plus.php?id=U45A snoRNABase entry for SNORD45A]
* [http://www-snorna.biotoul.fr/plus.php?id=U45B snoRNABase entry for SNORD45B]
* [http://www-snorna.biotoul.fr/plus.php?id=U45C snoRNABase entry for SNORD45C]