Bacteriophage pRNA

During replication of linear dsDNA viruses, the viral genome is packaged into the pre-formed viral procapsid. The packaging of DNA into the procapsid requires a molecular motor which using ATP as energy to accomplish the energetically unfavourable motion. In some bacteriophage, an RNA (pRNA) molecule is a vital component of this motor cite journal |author=Guo P |title=Structure and function of phi29 hexameric RNA that drives the viral DNA packaging motor: review |journal=Prog. Nucleic Acid Res. Mol. Biol. |volume=72 |issue= |pages=415–72 |year=2002 |pmid=12206459 |doi=] . Structural analyses of the packaging motor have demonstrated that the pRNA molecule has fivefold symmetry when attached to the prohead cite journal |author=Morais MC, Tao Y, Olson NH, "et al" |title=Cryoelectron-microscopy image reconstruction of symmetry mismatches in bacteriophage phi29 |journal=J. Struct. Biol. |volume=135 |issue=1 |pages=38–46 |year=2001 |pmid=11562164 |doi=10.1006/jsbi.2001.4379] cite journal |author=Simpson AA, Tao Y, Leiman PG, "et al" |title=Structure of the bacteriophage phi29 DNA packaging motor |journal=Nature |volume=408 |issue=6813 |pages=745–50 |year=2000 |pmid=11130079 |doi=10.1038/35047129] cite journal |author=Badasso MO, Leiman PG, Tao Y, "et al" |title=Purification, crystallization and initial X-ray analysis of the head-tail connector of bacteriophage phi29 |journal=Acta Crystallogr. D Biol. Crystallogr. |volume=56 |issue=Pt 9 |pages=1187–90 |year=2000 |pmid=10957642 |doi=] . The pRNA is thought to be bound by the capsid connector protein cite journal |author=Badasso MO, Leiman PG, Tao Y, "et al" |title=Purification, crystallization and initial X-ray analysis of the head-tail connector of bacteriophage phi29 |journal=Acta Crystallogr. D Biol. Crystallogr. |volume=56 |issue=Pt 9 |pages=1187–90 |year=2000 |pmid=10957642 |doi=] . Only the first 120 bases of the pRNA are essential for packing the viral DNA cite journal |author=Guo PX, Erickson S, Anderson D |title=A small viral RNA is required for in vitro packaging of bacteriophage phi 29 DNA |journal=Science |volume=236 |issue=4802 |pages=690–4 |year=1987 |pmid=3107124 |doi=] cite journal |author=Bailey S, Wichitwechkarn J, Johnson D, Reilly BE, Anderson DL, Bodley JW |title=Phylogenetic analysis and secondary structure of the Bacillus subtilis bacteriophage RNA required for DNA packaging |journal=J. Biol. Chem. |volume=265 |issue=36 |pages=22365–70 |year=1990 |pmid=2125049 |doi=] . The pRNA is proposed to be comprised of two domains, one corresponding to the first 120 bases and the second to the remaining 50 bases cite journal |author=Bailey S, Wichitwechkarn J, Johnson D, Reilly BE, Anderson DL, Bodley JW |title=Phylogenetic analysis and secondary structure of the Bacillus subtilis bacteriophage RNA required for DNA packaging |journal=J. Biol. Chem. |volume=265 |issue=36 |pages=22365–70 |year=1990 |pmid=2125049 |doi=] . Nuclear cleavage occurs in the single strand region linking these two domains cite journal |author=Bailey S, Wichitwechkarn J, Johnson D, Reilly BE, Anderson DL, Bodley JW |title=Phylogenetic analysis and secondary structure of the Bacillus subtilis bacteriophage RNA required for DNA packaging |journal=J. Biol. Chem. |volume=265 |issue=36 |pages=22365–70 |year=1990 |pmid=2125049 |doi=] .

References

External links

*