NeutrAvidin

NeutrAvidin protein is a deglycosylated version of avidin, with a mass of approximately 60,000 daltons. As a result of carbohydrate removal, lectin binding is reduced to undetectable levels, yet biotin binding affinity is retained because the carbohydrate is not necessary for this activity. NeutrAvidin has a near-neutral pI (6.3) that minimizes nonspecific interactions, along with lysine residues that remain available for derivatization or conjugation.

Like avidin itself, NeutrAvidin is a tetramer with a strong affinity for biotin (K_d = 10^-15 M). In biochemical applications, streptavidin, which also binds very tightly to Biotin, may be used interchangeably with NeutrAvidin.

Avidin immobilized onto solid supports is also used as purification media to capture biotin-labelled protein or nucleic acid molecules. For example, cell surface proteins can be specifically labelled with membrane impermeable biotin reagent, then specifically captured using a NeutrAvidin support.

References

1. Bayer, Ed: "The Avidin-Biotin Complex", Dept. of Biological Chemistry, Weizmann Institute of Science, Israel

This protein-related article is a stub. You can help Wikipedia by expanding it.v · Categories: Proteins Protein stubs Wikimedia Foundation. 2010. Игры ⚽ Нужен реферат? Stephan Bodecker Thomas Hubbard Look at other dictionaries: neutravidin — noun A deglycosylated version of avidin …   Wiktionary Avidin — Biological Overview= Avidin is a tetrameric protein produced in the oviducts of birds, reptiles and amphibians which is deposited in the whites of their eggs. In chicken egg white, avidin makes up approximately 0.05% of total protein… …   Wikipedia Biotin — Biotin[1] …   Wikipedia Streptavidin — is a 53,000 dalton [ [http://www.piercenet.com/Products/Browse.cfm?fldID=01021102 NeutrAvidin Agarose and other NeutrAvidin Beads ] ] tetrameric protein purified from the bacterium Streptomyces avidinii . It finds wide use in molecular biology… …   Wikipedia Streptavidine — Demande de traduction Streptavidin → Streptavidi …   Wikipédia en Français Biotinylation — In biochemistry, biotinylation is the process of covalently attaching a biotin tag to a molecule or surface. Labeling methodsPrimary amine biotinylationThe most common target for modifying protein molecules is amine groups, which are present as… …   Wikipedia Vitamine B8 — Traduction à relire Biotin → Vitamine B8 (+ …   Wikipédia en Français 18+ © Academic, 2000-2024 Contact us: Technical Support, Advertising Dictionaries export, created on PHP, Joomla, Drupal, WordPress, MODx. Mark and share Search through all dictionaries Translate… Search Internet Share the article and excerpts Direct link … Do a right-click on the link above and select “Copy Link”