- Neoschizomer
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Neoschizomers are restriction enzymes that recognize the same nucleotide sequence as their prototype but cleave at a different site. In some special applications this is a very helpful feature.
Recognition sequences and products of neoschizomers
For example:Prototype MaeII A^CGT produces DNA fragments with a 2-base 5' extension Neoschizomer TaiI ACGT^ produces DNA fragments with a 4-base 3' extension
Prototype ApaI GGGCC^C produces DNA fragments with a 4-base 3' extension Neoschizomer Bsp120I G^GGCCC produces DNA fragments with a 4-base 5' extension
There are also other pairs of neoschizomers.
The special class of neoschizomers that cleave at the same site are known as isoschizomers.
References
Rebase restriction enzyme database, http://rebase.neb.com/rebase/
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