Endonuclease

Endonuclease

Endonucleases are enzymes that cleave the phosphodiester bond within a polynucleotide chain, in contrast to exonucleases, which cleave phosphodiester bonds at the end of a polynucleotide chain. "Restriction endonucleases" (Restriction Enzymes) cleave DNA at specific sites, and are divided into three categories, Type I, Type II, and Type III, according to their mechanism of action. These enzymes are often used in genetic engineering to make recombinant DNA for introduction into bacterial, plant, or animal cells.

Common endonucleases:

Restriction endonucleases (ENases) are products of bacteria, and can be used to map a piece of DNA.

Bacterial:

# UvrABC endonuclease is a well documented endonuclease found in "E.coli".

ee also

* Exonuclease
* Nuclease
* Ribonuclease


Wikimedia Foundation. 2010.

Игры ⚽ Поможем написать реферат

Look at other dictionaries:

  • Endonuclease — Endonucléase Une endonucléase est une nucléase, qui coupe un acide nucléique en fragments plus courts. Les endonucléases sont capables de couper au milieu de la chaîne, par opposition aux exonucléases qui n attaquent que les nucléotides situés… …   Wikipédia en Français

  • endonuclease — endonuclease. См. эндонуклеаза. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • Endonucléase — Une endonucléase est une nucléase, qui coupe un acide nucléique en fragments plus courts. Les endonucléases sont capables de couper au milieu de la chaîne, par opposition aux exonucléases qui n attaquent que les nucléotides situés aux extrémités… …   Wikipédia en Français

  • Endonuclease — An enzyme that cleaves a nucleic acid (DNA or RNA) at specific internal sites in the nucleotide base sequence. * * * An enzyme (phosphodiesterase) that cleaves the internal phosphodiester bonds in a DNA molecule, thus producing DNA fragments of… …   Medical dictionary

  • endonuclease — endonukleazė statusas T sritis chemija apibrėžtis Nukleazė, katalizuojanti fosfodiesterinių ryšių, esančių polinukleotidinės grandinės viduje, hidrolizę. atitikmenys: angl. endonuclease rus. эндонуклеаза …   Chemijos terminų aiškinamasis žodynas

  • endonuclease — An enzyme that cleaves a phosphodiester bond within a DNA strand, forming two smaller strands. See: exonuclease, restriction endonuclease …   Glossary of Biotechnology

  • endonuclease — noun a nuclease that cleaves nucleic acids at interior bonds and so produces fragments of various sizes • Hypernyms: ↑nuclease • Hyponyms: ↑restriction endonuclease, ↑restriction nuclease, ↑restriction enzyme …   Useful english dictionary

  • Endonuclease/Exonuclease/phosphatase family — Pfam box Symbol = Exo endo phos Name = Endonuclease/Exonuclease/phosphatase family width = caption = Pfam= PF03372 InterPro= IPR005135 SMART= Prosite = PDOC00598 SCOP = 1ako TCDB = OPM family= 148 OPM protein= 1zwx PDB=PDB3|1i9yA:560 846… …   Wikipedia

  • endonuclease Serratia marcescens — A nuclease (a nucleate oligonucleotidohydrolase) that forms oligonucleotides ending in 5′ phosphates from RNA and DNA; hydrolyzes both double stranded and single stranded polynucleic acids. SYN: nucleate endonuclease …   Medical dictionary

  • endonuclease — noun Date: 1962 an enzyme that breaks down a nucleotide chain into two or more shorter chains by cleaving the internal covalent bonds linking nucleotides compare exonuclease …   New Collegiate Dictionary

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”