Insertional inactivation

Insertional inactivation

Insertional inactivation is a technique used in recombinant DNA engineering where a plasmid (such as pBR322) [ [http://www.uark.edu/campus-resources/mivey/m4233/recdna.html Recombinant DNA ] ] is used to disable expression of a gene. [ [http://www.cartage.org.lb/en/themes/Reference/dictionary/Biologie/I/125.html Insertional inactivation ] ]

The inactivation of a gene by inserting a fragment of DNA into the middle of its coding sequence. Any future products from the inactivated gene will not work because of the extra codes added to it.

References

External links

* [http://content.gene-technology-online.com/genlib/selection.htm Diagram at gene-technology-online.com]


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  • инактивация вставкой — Прекращение экспрессии гена в результате вставки; метод И.в. используется для выявления рекомбинантной ДНК при клонировании в плазмидных векторах по прекращению экспрессии селектируемого маркера. [Арефьев В.А., Лисовенко Л.А. Англо русский… …   Справочник технического переводчика

  • Инсерционная инактивация — * інсерцыйная інактывацыя * insertional inactivation нарушение кодирующей области гена путем инсерции (включения, вставки) чужеродной ДНК, ведущее к потере функции гена, т. е. к прекращению его экспрессии. И. и. позволяет легко отбирать… …   Генетика. Энциклопедический словарь

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