OPSI may stand for:
- Overwhelming post-splenectomy infection, rapidly fatal septicaemia in a patient who has undergone splenectomy (removal of the spleen).
- Office of Public Sector Information, a UK government body incorporating Her Majesty's Stationery Office.
- Open PC Server Integration An open source systems management system with the emphasis on automating software deployment on Windows clients, based on a Linux server.
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