- Ada (protein)
Ada is an
enzyme induced by treatment of bacterial cells withalkylating agents that mainly causemethylation damage. This phenomenon is called theadaptive response and hence the name. Ada is also called as O6 alkyl guanine transferase I (O6 AGT I) . Ada transfers the alkyl group fromDNA bases and sugar-phosphate backbone to a cysteine residue inactivating itself. Consequently, it reactsstoichiometrically with its substrate rather than catalytically and is referred to as a suicide enzyme. Methylation of Ada protein converts it into a self transcriptional activator, inducing its own gene expression and the expression of other genes which together with Ada help the cells repair alkylation damage [2] . Ada removes the alkyl group attached to DNA bases likeguanine (O6-alkyl guanine) orthymine (O4-alkyl thymine) and to the oxygen of the phosphodiester backbone of the DNA [1,3] . However, Ada shows greater preference for O6- alkyl guanine compared to either O4-thymine and alkylated phosphotriesters. Ada enzyme has two active sites, one for the alkylated guanines and thymines and the other for alkylated phosphotriesters [2] .References
# Errol C. Friedberg, Graham C. Walker, Wolfram Siede,Richard D. Wood , Roger A. Schultz, Tom Ellenberger, DNA Repair and Mutagenesis, second edition, ASM press, ISBN 1-55581-319-4
# Lindahl,T.,B. Sedgwick, M.Sekiguchi, & Y.Nakabeppu.(1988) Regulation and expression of adaptive response to alkylating agents, annu.Rev.Biochem.57:133-157
# McCarthy,T.V., and T. Lindahl.(1985)Methyl phosphotriesters in alkylated DNA are repaired by the Ada regulatory protein of E.Coli, Nucleic Acids Res.13:2683-2698
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