HaeIII

HaeIII

HaeIII is one of the 100+ restriction enzymes (endonucleases) discovered since 1970. It was isolated from the "Haemophilus aegyptius" bacteria, and has a molecular weight of 37126. The enzyme's recognition site—the place where it cuts DNA molecules—is the GGCC nucleotide sequence. This enzyme's gene has been sequenced and cloned.

Mechanism of action

The enzyme cleaves the DNA at the positions where the GGCC sequence is found. The cleavage occurs between the second and the third nucleotides (G and C). The resulting DNA fragments are known as restriction fragments. HaeIII cuts both strands of DNA in the same location, yielding restriction fragments with blunt ends.

ources

*Rawn, J. David (1989). "Biochemistry, International Edition". ISBN 0-89278-405-9
* [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?val=2961232&itemID=2&view=gbwithparts NCBI Sequence Viewer] .


Wikimedia Foundation. 2010.

Игры ⚽ Нужно сделать НИР?

Look at other dictionaries:

  • HaeIII — Masse/Länge Primärstruktur 318 Aminosäuren …   Deutsch Wikipedia

  • HaeIII — Secuencia de la enzima HaeIII. HaeIII es una enzima de restricción producida por el microorganismo Haemophilus aegyptius que posee una diana de restricción en el ADN de cadena doble dependiente de una secuencia no metilada, palindrómica y no… …   Wikipedia Español

  • Methyltransferase — HaeIII methyltransferase / DNA. Rendered from PDB 1DCT …   Wikipedia

  • In vitro compartmentalization — (IVC) is an emulsion based technology that generates cell like compartments in vitro . These compartments are designed such that each contains no more than one gene. When the gene is transcribed and/or translated, its products (RNAs and/or… …   Wikipedia

  • PRH1 — Proline rich protein HaeIII subfamily 1, also known as PRH1, is a human gene.cite web | title = Entrez Gene: PRH1 proline rich protein HaeIII subfamily 1| url = http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene Cmd=ShowDetailView… …   Wikipedia

  • Enzima de restricción — Saltar a navegación, búsqueda Enzima de Restricción EcoRI unida al ADN Una enzima de restricción (o endonucleasas de Restricción) es una enzima que puede reconocer una secuencia característica de nucleótidos dentro de una molécula de ADN y cortar …   Wikipedia Español

  • Restriction enzyme — Glossary Restriction …   Wikipedia

  • Classification of indigenous peoples of the Americas — Cultural regions of North American people at the time of European contact …   Wikipedia

  • Use of biotechnology in pharmaceutical manufacturing — Escherichia coli bacteria, which are often utilized in production of pharmaceutical products. Modern pharmaceutical manufacturing techniques frequently rely upon biotechnology. Contents 1 …   Wikipedia

  • Indigenous peoples of the Pacific Northwest Coast — This article is about the indigenous peoples of the Pacific Northwest Coast. For other indigenous peoples see Indigenous peoples (disambiguation) Chief Anotklosh of the Taku Tribe of the Tlingit people, ca. 1913 The Indigenous peoples of the… …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”