Antiseptics (from Greek "αντί" - "anti", '"against" + "σηπτικός" - "septikos", "putrefactive") are antimicrobial substances that are applied to living tissue/
skinto reduce the possibility of infection, sepsis, or putrefaction. They should generally be distinguished from " antibiotics" that destroy bacteriawithin the body, and from " disinfectants", which destroy microorganisms found on non-living objects. Some antiseptics are true " germicides", capable of destroying microbes ( bacteriocidal), whilst others are bacteriostaticand only prevent or inhibit their growth. Antibacterialsare antiseptics that only act against bacteria.
Usage in surgery
The widespread introduction of antiseptic surgical methods followed the publishing of the paper "
Antiseptic Principle of the Practice of Surgery" in 1867by Joseph Lister, inspired by Louis Pasteur's germ theory of putrefaction. In this paper he advocated the use of carbolic acid ( phenol) as a method of ensuring that any germs present were killed. Some of this work was anticipated by:
George H Tichenorwho experimented with the use of alcohol on wounds ca. 1861-1863, and subsequently marketed a product for this purpose known as "Dr. Tichenor's Patent Medicine " after the American Civil War.
Ignaz Semmelweiswho published his work "The Cause, Concept and Prophylaxis of Childbed Fever" in 1861, summarizing experiments and observations since 1847. [cite journal |author=Best M, Neuhauser D |title=Ignaz Semmelweis and the birth of infection control |journal=Qual Saf Health Care |volume=13 |issue=3 |pages=233–4 |year=2004 |pmid=15175497 |pmc=1743827 |doi=10.1136/qhc.13.3.233 |url=]
Florence Nightingale, who contributed substantially to the report on the Royal Commission on the Health of the Army (1856–1857), based on her earlier work
Oliver Wendell Holmes, Sr., who published "The Contagiousness of Puerperal Fever" in 1843. and even the ancient Greek physicians Galen(ca 130–200 AD) and Hippocrates(ca 400 BC). There is even a Sumerianclay tablet dating from 2150 BC advocating the use of similar techniques. [cite journal |author=Eming SA, Krieg T, Davidson JM |title=Inflammation in wound repair: molecular and cellular mechanisms |journal=J. Invest. Dermatol. |volume=127 |issue=3 |pages=514–25 |year=2007 |pmid=17299434 |doi=10.1038/sj.jid.5700701 |url=]
But every antiseptic, however good, is more or less toxic and irritating to a wounded surface. Hence it is that the antiseptic method has been replaced in the
surgeryof today by the aseptic method, which relies on keeping free from the invasion of bacteria rather than destroying them when present.
How does it work?
For the growth of bacteria there must be a certain food supply, moisture, in most cases oxygen, and a certain minimum temperature (see
bacteriology). These conditions have been specially studied and applied in connection with the preserving of food and in the ancient practice of embalmingthe dead, which is the earliest illustration of the systematic use of antiseptics.
In early inquiries a great point was made of the prevention of putrefaction, and work was done in the way of finding how much of an agent must be added to a given solution, in order that the bacteria accidentally present might not develop. But for various reasons this was an inexact method, and today an antiseptic is judged by its effects on pure cultures of definite pathogenic celicular single helix microbes, and on their vegetative and spore forms. Their standardization has been affected in many instances, and a water solution of
phenolof a certain fixed strength is now taken as the standard with which other antiseptics are compared.
ome common antiseptics
Alcohols: Most commonly used are ethanol(60-90%), 1- propanol(60-70%) and 2-propanol/ isopropanol(70-80%) or mixtures of these alcohols. They are commonly referred to as "surgical alcohol". Used to disinfect the skin before injections are given, often along with iodine ( tincture of iodine) or some cationic surfactants ( benzalkonium chloride0.05 - 0.5%, chlorhexidine0.2 - 4.0% or octenidine dihydrochloride 0.1 - 2.0%).
*;Quaternary ammonium compounds: Also known as "Quats" or "QAC's", include the chemicals
benzalkonium chloride(BAC), cetyl trimethylammonium bromide(CTMB), cetylpyridinium chloride(Cetrim, CPC) and benzethonium chloride(BZT). Benzalkonium chloride is used in some pre-operative skin disinfectants (conc. 0.05 - 0.5%) and antiseptic towels. The antimicrobial activity of Quats is inactivated by anionic surfactants, such as soaps. Related disinfectants include chlorhexidineand octenidine.
Boric acid: Used in suppositories to treat yeast infections of the vagina, in eyewashes, and as an antiviral to shorten the duration of cold soreattacks. Put into creams for burns. Also common in trace amounts in eye contact solution. Though it is popularly known as an antiseptic, it is in reality only a soothing fluid, and bacteria will flourish comfortably in contact with it.Fact|date=September 2008
Chlorhexidine Gluconate: A biguanidine derivative, used in concentrations of 0.5 - 4.0% alone or in lower concentrations in combination with other compounds, such as alcohols. Used as a skin antiseptic and to treat inflammation of the gums ( gingivitis). The microbicidal action is somewhat slow, but remanent. It is a cationic surfactant, similar to Quats.
Hydrogen peroxide: Used as a 6% (20Vols) solution to clean and deodorize wounds and ulcers. More common 1% or 2% solutions of hydrogen peroxide have been used in household first aid for scrapes, etc. However, even this less potent form is no longer recommended for typical wound care as the strong oxidization causes scar formation and increases healing time. Gentle washing with mild soap and water or rinsing a scrape with sterile saline is a better practice.
Iodine: Usually used in an alcoholic solution (called tincture of iodine) or as Lugol's iodinesolution as a pre- and post-operative antiseptic. No longer recommended to disinfect minor wounds because it induces scar tissue formation and increases healing time. Gentle washing with mild soap and water or rinsing a scrape with sterile saline is a better practice. Novel iodine antiseptics containing povidone-iodine(an iodophor, complex of povidone, a water-soluble polymer, with triiodide anions I3-, containing about 10% of active iodine) are far better tolerated, don't affect wound healing negatively and leave a deposit of active iodine, creating the so-called "remanent," or persistent, effect. The great advantage of iodine antiseptics is the widest scope of antimicrobial activity, killing all principal pathogenes and given enough time even spores, which are considered to be the most difficult form of microorganisms to be inactivated by disinfectants and antiseptics.
Mercurochrome: Not recognized as safe and effective by the U.S. Food and Drug Administration(FDA) due to concerns about its mercury content. Other obsolete organomercury antiseptics include bis-(phenylmercuric) monohydrogenborate (Famosept).
Octenidinedihydrochloride: A cationic surfactant and bis-(dihydropyridinyl)-decane derivative, used in concentrations of 0.1 - 2.0%. It is similar in its action to the Quats, but is of somewhat broader spectrum of activity. Octenidine is currently increasingly used in continental Europe as a QAC's and chlorhexidine (with respect to its slow action and concerns about the carcinogenicimpurity 4-chloroaniline) substitute in water- or alcohol-based skin, mucosa and wound antiseptic. In aqueous formulations, it is often potentiated with addition of 2-phenoxyethanol.
Phenol(carbolic acid) compounds: Phenol is germicidal in strong solution, inhibitory in weaker ones. Used as a "scrub" for pre-operative hand cleansing. Used in the form of a powder as an antiseptic baby powder, where it is dusted onto the navelas it heals. Also used in mouthwashes and throat lozenges, where it has a painkilling effect as well as an antiseptic one. Example: TCP. Other phenolic antiseptics include historically important, but today rarely used (sometimes in dental surgery) thymol, today obsolete hexachlorophene, still used triclosanand sodium 3,5-dibromo-4-hydroxybenzenesulfonate (Dibromol).
Sodium chloride: Used as a general cleanser. Also used as an antiseptic mouthwash. Only a weak antiseptic effect, due to hyperosmolalityof the solution above 0.9%.
Sodium hypochlorite: Used in the past, diluted, neutralized and combined with potassium permanganatein the Daquin's solution. It is now used only as disinfectant.
Stuart B. Levy, in a presentation to the 2000 Emerging Infectious Diseases Conference, expressed concern that the overuse of antiseptic and antibacterial agents might lead to an increase in dangerous, resistant strains of bacteria. [http://www.cdc.gov/ncidod/eid/vol7no3_supp/levy.htm] Dubious|date=September 2008
The body produces its own antiseptics, which are a part of the
chemical barriersof the immune system. The skin and respiratory tract secrete antimicrobial peptidessuch as the β-defensins. [cite journal |author=Agerberth B, Gudmundsson GH |title=Host antimicrobial defence peptides in human disease |journal=Curr. Top. Microbiol. Immunol. |volume=306 |issue= |pages=67–90 |year=2006 |pmid=16909918 |doi= |url=] Enzymes such as lysozymeand phospholipase A2in saliva, tears, and breast milkare also antiseptic. [cite journal | author = Moreau J, Girgis D, Hume E, Dajcs J, Austin M, O'Callaghan R |title=Phospholipase A(2) in rabbit tears: a host defense against Staphylococcus aureus. | url=http://www.iovs.org/cgi/content/full/42/10/2347 |journal=Invest Ophthalmol Vis Sci |volume=42 |issue=10 |pages=2347–54 |year=2001 |pmid= 11527949] [cite journal | author = Hankiewicz J, Swierczek E |title=Lysozyme in human body fluids. |journal=Clin Chim Acta |volume=57 |issue=3 |pages=205–9 |year=1974 |pmid= 4434640 | doi = 10.1016/0009-8981(74)90398-2] Vaginal secretions serve as a chemical barrier following menarche, when they become slightly acidic, while semencontains defensins and zincto kill pathogens. [cite journal | author = Fair W, Couch J, Wehner N |title=Prostatic antibacterial factor. Identity and significance. |journal=Urology |volume=7 |issue=2 |pages=169–77 |year=1976 |pmid= 54972 | doi = 10.1016/0090-4295(76)90305-8] [cite journal | author = Yenugu S, Hamil K, Birse C, Ruben S, French F, Hall S |title=Antibacterial properties of the sperm-binding proteins and peptides of human epididymis 2 (HE2) family; salt sensitivity, structural dependence and their interaction with outer and cytoplasmic membranes of Escherichia coli. | url= |journal=Biochem J |volume=372 |issue=Pt 2 |pages=473–83 |year=2003 |pmid= 12628001 |pmc=1223422 |doi = 10.1042/BJ20030225] In the stomach, gastric acidand proteases serve as powerful chemical defenses against ingested pathogens.
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