Blot (biology)

Blot (biology)

In molecular biology and genetics, a blot is a method of transferring proteins, DNA or RNA, onto a carrier (for example, a nitrocellulose PVDF or nylon membrane). In many instances, this is done after a gel electrophoresis, transferring the molecules from the gel onto the blotting membrane, and other times adding the samples directly onto the membrane. After the blotting, the transferred proteins, DNA or RNA are then visualized by one or more different methods:
* colorant staining (for example, silver staining of proteins)
* autoradiographic visualization of radioactive labelled molecules (performed before the blot)
* specific labelling of some proteins or nucleic acids. It is done with antibodies or hybridization probes that bind only to some molecules of the blot and have an enzyme joined to them. After proper washing, this enzymatic activity (and so, the molecules we search in the blot) is visualized by incubation with proper reactive, rendering either a colored deposit on the blot or a chemiluminiscent reaction which is registered by photographic film.

Common blot methods are :
* Southern blot for DNA
* Northern blot for RNA
* Western blot for proteins
* Southwestern blot
* Dot blot
* Slot blot

External links

* [http://www.westernblotting.org Western Blot Protocols and Troubleshooting]


Wikimedia Foundation. 2010.

Игры ⚽ Нужно сделать НИР?

Look at other dictionaries:

  • Blot — A blot can refer to several different things. *In biology, a blot is a method of transferring proteins, DNA, RNA or a protein onto a carrier. The following types are known: **Southern blot to recognize DNA **Northern blot to recognize RNA… …   Wikipedia

  • Blot, Southern — A test commonly used in molecular biology and genetics, the purpose of the test being to check for a match between DNA molecules. In the Southern blot technique, DNA fragments are separated by a method called agarose gel electrophoresis,… …   Medical dictionary

  • Blot, Northern — A technique in molecular biology, used mainly to separate and identify pieces of RNA. Called a Northern blot only because it is similar to a Southern blot (which is named after its inventor, the British biologist M.E. Southern). There is also a… …   Medical dictionary

  • Blot, Western — A technique in molecular biology, used to separate and identify proteins. Called a Western blot merely because it has some similarity to a Southern blot (which is named after its inventor, the British biologist M.E. Southern). There is also a… …   Medical dictionary

  • Molecular biology — (pronounced /məˈlɛkjʊlər .../) is the branch of biology that deals with the molecular basis of biological activity. This field overlaps with other areas of biology and chemistry, particularly genetics and biochemistry. Molecular biology… …   Wikipedia

  • Northern blot — The northern blot is a technique used in molecular biology research to study gene expression by detection of RNA (or isolated mRNA) in a sample.[1][2] …   Wikipedia

  • Dot blot — Schematic of the use of two ASO probes on duplicate Dot blot filters. A Dot blot (or Slot blot) is a technique in molecular biology used to detect biomolecules. It represents a simplification of the northern blot, Southern blot, or western blot… …   Wikipedia

  • Western blot — Resultado del Western blot: una membrana con las proteínas separadas en la electroforesis unidas, de las que sólo se disciernen aquellas contra las se ha añadido un anticuerpo. El Western blot, o inmunoblot, es una técnica analítica usada para… …   Wikipedia Español

  • Portal:Biology — Wikipedia portals: Culture Geography Health History Mathematics Natural sciences People Philosophy Religion Society Technology  Science …   Wikipedia

  • List of molecular biology topics — This is a list of topics in molecular biology. See also list of biochemistry topics. NOTOC # 3 end 3 flanking region 5 end 5 flanking region 5 ribose 3 A acrylamide gels adenine adenosine deaminase deficiency adenovirus agarose gel… …   Wikipedia

Share the article and excerpts

Direct link
… Do a right-click on the link above
and select “Copy Link”