Explant culture

Explant culture

Explant culture is a technique used for the isolation of cells from a piece or pieces of tissue. Tissue harvested in this manner is called an "explant". It can be a portion of the shoot, leaves, or some cells from a plant, and can be any part of the tissue from an animal.

In brief, the tissue is harvested in a sterile manner, often minced, and pieces placed in a cell culture dish containing growth media. Over time, progenitor cells migrate out of the tissue onto the surface of the dish. These "primary cells" can then be further expanded and transferred into fresh dishes.

"Explant culture" can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding extracellular matrix to more accurately mimic the in vivo environment e.g. cartilage explant culture. [Orth MW et al. (2000) Cartilage turnover in embryonic chick tibial explant cultures. Poult Sci 79:990-993.]

References


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  • explant — [ ɛksplɑ̃ ] n. m. • v. 1937; de 1. ex et plant, d apr. implant ♦ Biol. Fragment d organe ou de tissu prélevé sur un organisme et cultivé in vitro. ● explant nom masculin (anglais explant, du latin explantare, déraciner) Fragment d organisme… …   Encyclopédie Universelle

  • Explant de base — Explant Dans le domaine du clonage et plus généralement des biotechnologies et de la production végétale, un « explant » est le matériel végétal de base qui servira à produire des clones végétaux à partir d une « plante mère » …   Wikipédia en Français

  • explant — [eks plant′] vt. [ EX 1 + PLANT, vt.] to transfer (living tissue) for culture in an artificial medium n. this tissue or culture explantation [eks΄plan tā′shən] n …   English World dictionary

  • Explant — Dans le domaine du clonage et plus généralement des biotechnologies et de la production végétale, un « explant » est le matériel végétal de base qui servira à produire des clones végétaux à partir d une « plante mère » ou… …   Wikipédia en Français

  • culture — 1. culture [ kyltyr ] n. f. • 1509; a. fr. couture XIIe; lat. cultura I ♦ 1 ♦ Action de cultiver la terre; ensemble des opérations propres à tirer du sol les végétaux utiles à l homme et aux animaux domestiques. ⇒ agriculture. Culture d un champ …   Encyclopédie Universelle

  • explant — Living tissue transferred from an organism to an artificial medium for culture. * * * ex·plant ( )ek splant vt to remove (living tissue) esp. to a medium for tissue culture ex·plan·ta·tion .ek .splan tā shən n ex·plant ek .splant n …   Medical dictionary

  • explant — I. transitive verb Etymology: ex + plant (as in implant) Date: 1915 to remove (living tissue) especially to a medium for tissue culture • explantation noun II. noun Date: 1917 living tissue removed from an organism and placed in a medium for… …   New Collegiate Dictionary

  • explant — n. tissue that has been taken from a living organism or plant and placed in a culture medium v. take tissue from a living organism or plant and place it in a culture medium …   English contemporary dictionary

  • explant — n. 1) live tissue transferred from the body (or any organism) to a suitable artificial medium for culture. The tissue grows in the artificial medium and can be studied for diagnostic or experimental purposes. Tumour growths are sometimes examined …   The new mediacal dictionary

  • explant — explantation, n. v. /eks plant , plahnt /; n. /eks plant , plahnt /, v.t. 1. to take living material from an animal or plant and place it in a culture medium. n. 2. a piece of explanted tissue. [1570 80; < NL explantare. See EX 1, PLANT] * * * …   Universalium

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