Amido black 10B

Chembox new
ImageFile = Amido black.png ImageSize = 320px
IUPACName = 4-Amino-5-hydroxy-3- [(4-nitrophenyl)azo] -6- -(phenylazo)-2,7-Naphthalene disulfonic acid, disodium salt
OtherNames = Amidoschwarz
Naphthol blue black
Acid Black 1
Acidal Black 10B
Acidal Navy Blue 3BR
Naphthalene Black 10B (see )
Buffalo Black NBR
C.I. 20470
Section1 = Chembox Identifiers
CASNo = 1064-48-8
PubChem = 9566044
SMILES = OC1=C(C(N)=C(/N=N/C4=CC=C( [N+] ( [O-] )=O) C=C4)C(S(=O)(O [Na] )=O)=C3)C3=CC(S(=O) (O [Na] )=O)=C1/N=N/C2=CC=CC=C2
Section2 = Chembox Properties
C=22|H=14|N=6|Na=2|O=9|S=2
Appearance = Dark red to black
Density =
MeltingPt =
BoilingPt =
Solubility = ~ 30 g/l at 20 °C
Section7 = Chembox Hazards
MainHazards =
FlashPt =
Autoignition =
NFPA-H = 2
NFPA-F = 0
NFPA-R = 0
RPhrases = R20, R21, R33
SPhrases = S28A, S37, S45

Amido black 10B is an amino acid staining diazo dye used in biochemical research to stain for total protein on transferred membrane blots. It is also used in criminal investigations to detect blood present with latent fingerprints. It stains the proteins in blood a blue-black color. Amido Black can be either methanol or water based as it readily dissolves in both. With picric acid, in a van Gieson procedure, it can be used to stain collagen and reticulin.

External links

* [http://www.chemicalland21.com/specialtychem/finechem/AMIDO%20BLACK%2010B.htm www.chemicalland21.com]
* [http://ptcl.chem.ox.ac.uk/MSDS/AM/amido_black_10B.html MSDS at Oxford University]