Crude lysate

Crude lysate

A crude lysate is the solution produced when cells are destroyed by disrupting their cell membranes, often with detergent or other chaotropic agent, in a process known as cytolysis. This releases the contents within the cell. Crude cell lysates are routinely produced in biochemistry and cell biology laboratories during the process of protein purification, although purified cellular organelles can also be retrieved from the solution. After a crude lysate has been generated, the first step in processing a crude lysate is often ultracentrifugation, which separates the solution into distinct bands containing organelles, membrane lipids, proteins, and nucleic acids. The crude lysate can also be analyzed directly without further processing or purification by such methods as Coomassie blue staining of polyacrylamide gels or Western blotting.

Wikimedia Foundation. 2010.

Игры ⚽ Нужно сделать НИР?

Look at other dictionaries:

  • Immunoprécipitation — L immunoprécipitation (IP) est la technique qui permet la précipitation d un antigène (protéine) en solution par un anticorps qui agglutine spécifiquement une protéine particulière. On l utilise pour isoler et concentrer une protéine précise… …   Wikipédia en Français

  • Immunoprecipitation — (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many… …   Wikipedia

  • Protein — Proteins are large organic compounds made of amino acids arranged in a linear chain and joined together by peptide bonds between the carboxyl and amino groups of adjacent amino acid residues. The sequence of amino acids in a protein is defined by …   Wikipedia

  • N-acyl phosphatidylethanolamine-specific phospholipase D — N acyl phosphatidylethanolamine phospholipase D Identifiers Symbol NAPEPLD Entrez 222236 HUGO …   Wikipedia

  • Methods to investigate protein–protein interactions — There are many methods to investigate protein–protein interactions. Each of the approaches has its own strengths and weaknesses, especially with regard to the sensitivity and specificity of the method. A high sensitivity means that many of the… …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”