A1 broth

A1 broth

An A1 broth is a liquid culture medium used in microbiology for the detection of fecal coliforms in foods, treated wastewater and seawater bays using the most probable number (MPN) method. It is prepared according to the formulation of Andrews and Presnell given below. [ [http://www.123exp-biology.com/t/01174245593/ A1 broth - Biology Dictionary and Research Guide ] ] It is used with a Durham tube, a positive tube being one that exhibits a trapped bubble of gas.

Typical formula (g/l) [ [http://www.sigmaaldrich.com/catalog/search/ProductDetail/FLUKA/17112 17112 A1 Broth BioChemika, for microbiology ] ]


=Directions [ [http://www.srlchem.com/products/product_details.php?productId=3649 Sisco Research Laboratories Pvt. Ltd ] ] =

Suspend the dry ingredients in one liter of cold distilled water. Gently heat until completely dissolved and distribute 9 ml into test tubes with an inverted Durham tube. Sterilize in an autoclave at 121°C for 15 minutes. If needed, prepare multi-strength broth weighing the appropriate quantity of the dry medium. The final pH is 6.9 ± 0.1.

Widespread usage

Variants of this test has been used for potable water across the globe, for example by the Cree community of Split Lake, Manitoba, by the Mapuche people of Maquehue, Chile [ [http://www.idrc.ca/en/ev-26962-201-1-DO_TOPIC.html Portable Water-testing Kit: International Development Research Centre ] ] and in Singapore, Malaysia and Thailand [ [http://www3.interscience.wiley.com/cgi-bin/abstract/112657962/ABSTRACT?CRETRY=1&SRETRY=0] ]

References


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Look at other dictionaries:

  • Broth — Broth, n. [AS. bro[eth]; akin to OHG. brod, brot; cf. Ir. broth, Gael. brot. [root]93. Cf. {Brewis}, {Brew}.] Liquid in which flesh (and sometimes other substances, as barley or rice) has been boiled; thin or simple soup. [1913 Webster] I am sure …   The Collaborative International Dictionary of English

  • broth — [brɔθ US bro:θ] n [U and C] [: Old English;] soup with meat, rice, or vegetables ▪ chicken broth →↑Scotch broth …   Dictionary of contemporary English

  • broth|el — «BROTH uhl, BROTH ; BRTH , BRTH », noun. a house of prostitution. ╂[Middle English brothel abandoned person < Old English brēothan go to ruin] …   Useful english dictionary

  • broth — brȯth n, pl broths brȯths, brȯthz 1) liquid in which meat or sometimes vegetable food has been cooked 2) a fluid culture medium * * * (broth) 1. a thin soup prepared by boiling meat or vegetables. 2. a liquid culture medium for the cultivation …   Medical dictionary

  • broth — [ brɔθ ] noun uncount 1. ) OLD FASHIONED soup, especially when it is considered to be good for you: beef broth 2. ) a liquid used for giving flavor when cooking …   Usage of the words and phrases in modern English

  • broth — [brôth, bräth] n. [ME & OE; akin to OHG brod: for IE base see BREAD] a clear, thin soup made by boiling meat, or sometimes cereals or vegetables, in water …   English World dictionary

  • broth|er-in-law — «BRUHTH uhr ihn L», noun, plural broth|ers in law. 1. the brother of one s husband or wife: »She asked her brother in law if he could come for his brother s birthday. 2. the husband of one s sister: »He called his sister to see when his brother… …   Useful english dictionary

  • broth|er — «BRUHTH uhr», noun, plural broth|ers or (Archaic) breth|ren, adjective, verb. –n. 1. a) the son of the same parents. A boy is a brother to the other children of his parents. »He is in a family of four brothers. b) the son only of the same mother… …   Useful english dictionary

  • broth — O.E. broþ, from P.Gmc. *bruthan (Cf. O.H.G. *brod), from verb root *bhreue to heat, boil, bubble, liquid in which something has been boiled (Cf. O.E. breowan to brew; see BREW (Cf. brew)). Picked up from Germanic by the Romanic and Celtic… …   Etymology dictionary

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  • broth — ► NOUN 1) soup consisting of meat or vegetable chunks cooked in stock. 2) a liquid nutrient medium for the culture of bacteria. ORIGIN Old English, related to BREW(Cf. ↑brewer) …   English terms dictionary

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