Pilobolus

:"This article is about the fungal genus; for information on the dance company, see "Pilobolus (dance company)".

Taxobox |

name = "Pilobolus"
regnum = Fungi
phylum = Zygomycota
classis = Zygomycetes
ordo = Mucorales
familia = Pilobolaceae
genus = "Pilobolus"
subdivision_ranks = Species
subdivision = (incomplete list)
"P. crystallinus"
"P. kleinii"
"P. longipes"
"P. sphaerosporus"
"P. umbonatus"
"P. roridus"

"Pilobolus" is a genus of fungi that commonly grows on herbivore dung.

Life cycle

The life cycle of "Pilobolus" begins with a black sporangium that has been discharged onto a plant substrate such as grass. A herbivorous animal such as a horse then eats the substrate, unknowingly consuming the sporangium as well. The "Pilobolus" sporangium survives the passage through the gastrointestinal tract without germinating, and emerges with the excrement. Once outside its host, spores within the sporangium germinate and grow as a mycelium within the excrement, where it is a primary colonizer. Later, the fungus fruits to produce more spores.

The asexual fruiting structure (the sporangiophore) of "Pilobolus" species is unique. It consists of a transparent stalk which rises above the excrement to end in a balloon-like subsporangial vesicle. On top of this, a single, black sporangium develops. The sporangiophore has the remarkable ability of orienting itself to point directly towards a light source. The subsporangial vesicle acts as a lens, focusing light via carotenoid pigments deposited near the base of the vesicle. The developing sporangiophore grows such that the maturing sporangium is aimed directly at the light. When turgor pressure within the subsporangial vesicle builds to a sufficient level, (often 7 ATM or greater) the sporangium is launched, and can travel anywhere from a couple of centimeters to an incredible distance of 2 meters (6 feet), an amazing achievement for a sporangiophore less than 1 cm tall. The orientation of the stalk towards the light apparently guarantees that the sporangium is shot some distance from the excrement, enhancing the chances that it will attach to vegetation and be eaten by a new host.

Another interesting adaptation of "Pilobolus" is that the sporangium is covered in calcium oxalate crystals. Besides serving as a protective mechanism, their hydrophobic nature also leads the sporangium to flip over onto its sticky bottom after landing in a drop of dew, thus allowing it to cling to a plant grown in artificial culture, but only when the growth medium is supplemented with some form of chelated iron, or with sterilized herbivore dung.

The forcible discharge mechanism of "Pilobolus" is exploited by parasitic nematodes including lungworms in the genus "Dictyocaulus". Larval lungworm nematodes excreted by infected deer, elk, cattle, horses, and other hosts climb up "Pilobolus" sporangiophores and are discharged with the sporangium. They complete their life cycle when they and their "Pilobolus" vector are eaten by a new host.

References

*Bruce, V. G., F. Weight, and C. S. Pittendrigh. 1960. Resetting the sporulation rhythm in "Pilobolus" with short light flashes of high intensity. Science 131:728-730.
*Foos, K. M. 1997. "Pilobolus" and lungworm disease affecting elk in Yellowstone National Park. Mycological Research 101:1535-1536.
* Yafetto, L., Carroll, L., Cui, Y., Davis, D. J., Fischer, M. W., Henterly, A. C., Kessler, J. D., Kilroy, H. A., Shidler, J. B., Stolze-Rybczynski, J. L., Sugawara, Z., Money, N. P. 2008. The fastest flights in nature: high-speed spore discharge mechanisms among fungi. PLOS One 3:e3237.
*Uebelmesser, E. R. 1954. Ãœber den endogenen Tagesrhythmus der Sporangienbildung von "Pilobolus". Arch Mikrobiol 20:1-33.

External links

* [http://botit.botany.wisc.edu/toms_fungi/mar2006.html Pilobolus crystallinus, "The Fung in the Dung"] by Tom Volk
* [http://hosts.cce.cornell.edu/mushroom_blog/?p=106 Pilobolus and the Lungworm] at the Cornell Mushroom Blog (with time lapse video)
* [http://www.alaskawriters.com/dianestory.html Interview with a Fungus]
* [http://www.zygomycetes.org/index.php?id=77 Zygomycetes:Pilobolus] at Zygomycetes.org