Calcein

Calcein

Chembox new
Name = Calcein
ImageFile = Calcein.svg
ImageName =
IUPACName =
Section1 = Chembox Identifiers
CASNo = 1461-15-0
SMILES = O=C(O5)c1ccccc1C (c3cc(CN(CC(O)=O) CC(O)=O)c(O)cc3O4) 5c2c4cc(O)c(CN(CC (O)=O)CC(O)=O)c2

Section2 = Chembox Properties
Formula = C30H26N2O13
MolarMass = 622.55 g/mol
Density =
MeltingPt = Decomposes
BoilingPt = N/A

Calcein, also known as fluorexon, fluorescein complex, is a fluorescent dye with an excitation and emission wavelengths of 495/515nm, respectively. Calcein also self-quenches at concentrations above 100mM. It is used as a complexometric indicator for titration of calcium ions with EDTA, and for fluorometric determination of calcium. It has the appearance of orange crystals.

Applications

The acetomethoxy derivate of calcein (calcein AM) is used in biology as it can be transported through the cellular membrane into live cells, which makes it useful for testing of cell viability and for short-term labeling of cells. The acetomethoxy group obscures the part of the molecule that chelates calcium. After transport into the cell, intracellular esterases remove the group, the molecule binds to calcium within cell (resulting in strong green fluorescence), and gets trapped inside. As dead cells lack active esterases, only live cells are labeled. [http://www.howstuffworks.com/light-microscope4.htm]

Calcein is also used for marking freshly hatched fish [ [http://www.gct.org.uk/text03.asp?PageId=331 Marking fry using calcein - The Game Conservancy Trust ] ] and for labeling of bones in live animals.

References


Wikimedia Foundation. 2010.

Игры ⚽ Поможем сделать НИР

Look at other dictionaries:

  • Calcein — Strukturformel Allgemeines Name Calcein Andere Namen Fluorexon …   Deutsch Wikipedia

  • Calcein-AM — Strukturformel Allgemeines Name Calcein Andere Namen Fluorexon Fluorescein Komplexon Fluorescein 2,7 bis(methyleniminodiessigsäure) …   Deutsch Wikipedia

  • Calcein AM — Strukturformel Allgemeines Name Calcein Andere Namen Fluorexon Fluorescein Komplexon Fluorescein 2,7 bis(methyleniminodiessigsäure) …   Deutsch Wikipedia

  • calcein — A calcium chelating agent that fluoresces brightly in the presence of bound calcium. The acetomethoxy derivative can be transported into live cells and the reagent is useful as a viability test and for short term marking of cells …   Dictionary of molecular biology

  • calcein — noun A fluorescent dye related to fluorescein and having the chemical formula CHNO; used in some contexts as a calcium indicator See Also: phenol …   Wiktionary

  • Fluorexon — Strukturformel Allgemeines Name Calcein Andere Namen Fluorexon Fluorescein Komplexon Fluorescein 2,7 bis(methyleniminodiessigsäure) …   Deutsch Wikipedia

  • Triarylmethanfarbstoff — Triphenylmethan Den Grundkörper für die Gruppe der Triphenylmethanfarbstoffe bildet das Triphenylmethan. Mindestens zwei der aromatischen Ringe tragen elektronenliefernde Substituenten (sog. Auxochrome). Triphenylmethanfarbstoffe finden… …   Deutsch Wikipedia

  • Triphenylmethanfarbstoff — Triphenylmethan Den Grundkörper für die Gruppe der Triphenylmethanfarbstoffe bildet das Triphenylmethan. Mindestens zwei der aromatischen Ringe tragen elektronenliefernde Substituenten (sog. Auxochrome). Triphenylmethanfarbstoffe finden… …   Deutsch Wikipedia

  • Triphenylmethanfarbstoffe — Triphenylmethan Den Grundkörper für die Gruppe der Triphenylmethanfarbstoffe bildet das Triphenylmethan. Mindestens zwei der aromatischen Ringe tragen elektronenliefernde Substituenten (sogenannte Auxochrome). Triphenylmethanfarbstoffe finden… …   Deutsch Wikipedia

  • Complexometric indicator — A complexometric indicator is an ionochromic dye that undergoes a definite color change in presence of specific metal ions.[1] It forms a weak complex with the ions present in the solution, which has a significantly different color from the form… …   Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”