Vesicular stomatitis virus

Taxobox | color=violet
name = "Vesicular stomatitis virus"


image_caption = TEM micrograph of VSV virions.
virus_group = v
ordo = "Mononegavirales"
familia = "Rhabdoviridae"
genus = "Vesiculovirus"
type_species = "Vesicular stomatitis Indiana virus"
subdivision_ranks = Species
subdivision = "Carajas virus
"Chandipura virus"
"Cocal virus"
"Isfahan virus"
"Maraba virus"
"Piry virus"
"Vesicular stomatitis Alagoas virus"
"Vesicular stomatitis New Jersey virus"

"Vesicular stomatitis virus" (VSV) is a virus in the family Rhabdoviridae; the well-known "Rabies virus" belongs to the same family. VSV can infect insects and mammals. It has particular importance to farmers in certain regions of the world where it can infect cattle. It is also a common laboratory virus used to study the properties of viruses in the Rhabdoviridae family, as well as to study viral evolution.

VSV is an arbovirus: natural VSV infections encompass two steps, cytolytic infections of mammalian hosts and transmission by insects. In insects, infections are non-cytolytic persistent.

Vesicular stomatitis virus (VSV) is the prototypic member of the Vesiculovirus genera of the Rhabdovirus family. The genome of the virus is a single molecule of negative-sense RNA that encodes five major proteins: glycoprotein (G), matrix protein (M), nucleoprotein, large protein (L) and phosphoprotein.

Half the genome encodes the L protein, which combines with the phosphoprotein to catalyze replication of the mRNA.

The G protein enables viral entry to the cell by mediating both virus attachment to the host cell and fusion of the viral envelope with the endosomal membrane following endocytosis.

The mRNA encoding the vesicular stomatitis virus M protein is 831 nucleotides long, and encodes a protein of 229 amino acids. The predicted M protein sequence does not contain any long hydrophobic or nonpolar domains that might promote membrane association. The protein is rich in basic amino acids and contains a highly basic amino terminal domain.

The VSVG does not follow the same path as most vesicles because transport of the G protein from the endoplasmic reticulum to the plasma membrane is interrupted by incubation at 15°C. Under this condition, the molecules accumulate in both the endoplasmic reticulum (ER) and a subcellular vesicle fraction of low density called the lipid-rich vesicle fraction. The material in the lipid-rich vesicle fraction appears to be a post-ER intermediate in the transport process to the plasma membrane (PM). When synthesized in polarized epithelial cells, the envelope glycoproteins hemagglutinin of VSVG are targeted to the apical and basolateral plasma membranes. VSVG is also a common coat protein for lentiviral vector expression systems used to introduce genetic material into "in vitro" systems or animal models. It has the advantage or allowing the virus to infect non-dividing cells like B cells "in vitro".

Recently VSV has been found to have oncolytic properties, capable of killing cancer cells in two-thirds of all patients.Fact|date=November 2007

Current Research

Research has established VSV to have oncolytic properties. Research is ongoing, and has shown VSV to reduce tumor size and spread in melanoma, lung cancer, colon cancer and certain brain tumors.

External links

* [http://www.labonsite.com/background.php?id=3 Vesicular Stomatitis Virus] from The Lab-On-Site Project.