XPB

XPB (Xeroderma Pigmentosum B) is an ATP dependent human DNA helicase that is a part of the TFIIH transcription factor complex.

tructure

The 3D structure of the archeael homologue of XPB has been solved by X-ray crystallography by Dr. John Tainer and his group at The Scripps Research Institute. [cite journal |author=Fan L, Arvai A, Cooper P, Iwai S, Hanaoka F, Tainer J |title=Conserved XPB core structure and motifs for DNA unwinding: implications for pathway selection of transcription or excision repair |journal=Mol Cell |volume=22 |issue=1 |pages=27–37 |year=2006 |pmid=16600867 |doi=10.1016/j.molcel.2006.02.017]

Function

XPB plays a significant role in normal basal transcription, transcription coupled repair (TCR), and nucleotide excision repair (NER). Purified XPB has been shown to unwind DNA with 3’-5’polarity.

Disorders

Mutations in XPB and other related complementation groups, XPA-XPG, leads to a number of genetic disorders such as Xeroderma Pigmentosum, Cockayne's syndrome, and Trichothiodystrophy.

ee also

* XP

References

Further reading

PBB_Further_reading
citations =
*cite journal | author=Jeang KT |title=Tat, Tat-associated kinase, and transcription. |journal=J. Biomed. Sci. |volume=5 |issue= 1 |pages= 24–7 |year= 1998 |pmid= 9570510 |doi=
*cite journal | author=Yankulov K, Bentley D |title=Transcriptional control: Tat cofactors and transcriptional elongation. |journal=Curr. Biol. |volume=8 |issue= 13 |pages= R447–9 |year= 1998 |pmid= 9651670 |doi=
*cite journal | author=Cleaver JE, Thompson LH, Richardson AS, States JC |title=A summary of mutations in the UV-sensitive disorders: xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy. |journal=Hum. Mutat. |volume=14 |issue= 1 |pages= 9–22 |year= 1999 |pmid= 10447254 |doi= 10.1002/(SICI)1098-1004(1999)14:1<9::AID-HUMU2>3.0.CO;2-6 |doilabel=10.1002/(SICI)1098-1004(1999)14:19::AID-HUMU23.0.CO;2-6
*cite journal | author=Ma L, Weeda G, Jochemsen AG, "et al." |title=Molecular and functional analysis of the XPBC/ERCC-3 promoter: transcription activity is dependent on the integrity of an Sp1-binding site. |journal=Nucleic Acids Res. |volume=20 |issue= 2 |pages= 217–24 |year= 1992 |pmid= 1741247 |doi=
*cite journal | author=Weeda G, Wiegant J, van der Ploeg M, "et al." |title=Localization of the xeroderma pigmentosum group B-correcting gene ERCC3 to human chromosome 2q21. |journal=Genomics |volume=10 |issue= 4 |pages= 1035–40 |year= 1991 |pmid= 1916809 |doi=
*cite journal | author=Weeda G, Ma LB, van Ham RC, "et al." |title=Structure and expression of the human XPBC/ERCC-3 gene involved in DNA repair disorders xeroderma pigmentosum and Cockayne's syndrome. |journal=Nucleic Acids Res. |volume=19 |issue= 22 |pages= 6301–8 |year= 1991 |pmid= 1956789 |doi=
*cite journal | author=Weeda G, van Ham RC, Masurel R, "et al." |title=Molecular cloning and biological characterization of the human excision repair gene ERCC-3. |journal=Mol. Cell. Biol. |volume=10 |issue= 6 |pages= 2570–81 |year= 1990 |pmid= 2111438 |doi=
*cite journal | author=Weeda G, van Ham RC, Vermeulen W, "et al." |title=A presumed DNA helicase encoded by ERCC-3 is involved in the human repair disorders xeroderma pigmentosum and Cockayne's syndrome. |journal=Cell |volume=62 |issue= 4 |pages= 777–91 |year= 1990 |pmid= 2167179 |doi=
*cite journal | author=Wang XW, Yeh H, Schaeffer L, "et al." |title=p53 modulation of TFIIH-associated nucleotide excision repair activity. |journal=Nat. Genet. |volume=10 |issue= 2 |pages= 188–95 |year= 1995 |pmid= 7663514 |doi= 10.1038/ng0695-188
*cite journal | author=Maxon ME, Goodrich JA, Tjian R |title=Transcription factor IIE binds preferentially to RNA polymerase IIa and recruits TFIIH: a model for promoter clearance. |journal=Genes Dev. |volume=8 |issue= 5 |pages= 515–24 |year= 1994 |pmid= 7926747 |doi=
*cite journal | author=Maruyama K, Sugano S |title=Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligoribonucleotides. |journal=Gene |volume=138 |issue= 1-2 |pages= 171–4 |year= 1994 |pmid= 8125298 |doi=
*cite journal | author=Drapkin R, Reardon JT, Ansari A, "et al." |title=Dual role of TFIIH in DNA excision repair and in transcription by RNA polymerase II. |journal=Nature |volume=368 |issue= 6473 |pages= 769–72 |year= 1994 |pmid= 8152490 |doi= 10.1038/368769a0
*cite journal | author=van Vuuren AJ, Vermeulen W, Ma L, "et al." |title=Correction of xeroderma pigmentosum repair defect by basal transcription factor BTF2 (TFIIH). |journal=EMBO J. |volume=13 |issue= 7 |pages= 1645–53 |year= 1994 |pmid= 8157004 |doi=
*cite journal | author=Schaeffer L, Moncollin V, Roy R, "et al." |title=The ERCC2/DNA repair protein is associated with the class II BTF2/TFIIH transcription factor. |journal=EMBO J. |volume=13 |issue= 10 |pages= 2388–92 |year= 1994 |pmid= 8194528 |doi=
*cite journal | author=Guzder SN, Sung P, Bailly V, "et al." |title=RAD25 is a DNA helicase required for DNA repair and RNA polymerase II transcription. |journal=Nature |volume=369 |issue= 6481 |pages= 578–81 |year= 1994 |pmid= 8202161 |doi= 10.1038/369578a0
*cite journal | author=Vermeulen W, Scott RJ, Rodgers S, "et al." |title=Clinical heterogeneity within xeroderma pigmentosum associated with mutations in the DNA repair and transcription gene ERCC3. |journal=Am. J. Hum. Genet. |volume=54 |issue= 2 |pages= 191–200 |year= 1994 |pmid= 8304337 |doi=
*cite journal | author=Scott RJ, Itin P, Kleijer WJ, "et al." |title=Xeroderma pigmentosum-Cockayne syndrome complex in two patients: absence of skin tumors despite severe deficiency of DNA excision repair. |journal=J. Am. Acad. Dermatol. |volume=29 |issue= 5 Pt 2 |pages= 883–9 |year= 1993 |pmid= 8408834 |doi=
*cite journal | author=Blau J, Xiao H, McCracken S, "et al." |title=Three functional classes of transcriptional activation domain. |journal=Mol. Cell. Biol. |volume=16 |issue= 5 |pages= 2044–55 |year= 1996 |pmid= 8628270 |doi=
*cite journal | author=Iyer N, Reagan MS, Wu KJ, "et al." |title=Interactions involving the human RNA polymerase II transcription/nucleotide excision repair complex TFIIH, the nucleotide excision repair protein XPG, and Cockayne syndrome group B (CSB) protein. |journal=Biochemistry |volume=35 |issue= 7 |pages= 2157–67 |year= 1996 |pmid= 8652557 |doi= 10.1021/bi9524124
*cite journal | author=Hwang JR, Moncollin V, Vermeulen W, "et al." |title=A 3' --> 5' XPB helicase defect in repair/transcription factor TFIIH of xeroderma pigmentosum group B affects both DNA repair and transcription. |journal=J. Biol. Chem. |volume=271 |issue= 27 |pages= 15898–904 |year= 1996 |pmid= 8663148 |doi=

External links

*

PBB_Controls
update_page = yes
require_manual_inspection = no
update_protein_box = yes
update_summary = no
update_citations = yes