- Selector-technique
The selector technique allows multiplex amplification of arbitrary sets of genomic sequences. Genomic
DNA is digested with restriction enzymes, circularized by hybridisation to selectors and subsequently attached to a vector sequence byligation . The procedure results in circularDNA molecules with an included general primer pair motif that can be used for amplification byPCR or RCA.The selector construct
A selector consists of two oligonucleotides, one Vector oligonucletide and one Selector probe. Together they form one Selector with target specific ends on each side of a general primer motif.
election mechanisms
* (I) A selector probe hybridizes with both ends of the selected target.
* (II) A selector probe hybridizes with one end to the 3’ end of the target and the other end to an internal sequence of the target. The protruding 5' end is cleaved off usingTaq polymerase .Publications
* [http://nar.oxfordjournals.org/cgi/content/full/33/8/e71 Demonstration of the selector method]
* [http://nar.oxfordjournals.org/cgi/content/full/33/8/e72 The PieceMaker software for designing selector experiments]
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