Selectable marker

Selectable marker

A selectable marker is a gene introduced into a cell, especially a bacterium or to cells in culture, that confers a trait suitable for artificial selection. They are a type of reporter gene used in laboratory microbiology, molecular biology, and genetic engineering to indicate the success of a transfection or other procedure meant to introduce foreign DNA into a cell. Selectable markers are often antibiotic resistance genes; bacteria that have been subjected to a procedure to introduce foreign DNA are grown on a medium containing an antibiotic, and those bacterial colonies that can grow have successfully taken up and expressed the introduced genetic material.

An alternative to a selectable marker is a screenable marker, which allows the researcher to distinguish between wanted and unwanted cells.

Examples of selectable markers include:
*the Abicr gene [Edited By C. Arias, B.S., C.P.T.]
*Neo gene from Tn5, which confers antibiotic resistance to geneticin [ [http://www.cellmigration.org/resource/komouse/images/mousefig2.pngCallmigration.org: Gene targeting] ]

References


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  • Selectable Marker — Als Selectable Marker wird in der Gentechnik ein Gen bezeichnet, das zusammen mit dem „gene of interest“, also dem eigentlich gewünschten Gen, in den veränderten Organismus eingebracht wird, um Individuen mit erfolgreicher Genveränderung erkennen …   Deutsch Wikipedia

  • selectable marker — A gene whose expression allows the identification of a specific trait or gene in an organism …   Glossary of Biotechnology

  • dominant selectable marker — A gene that allows the host cell to survive under conditions where it would otherwise die. Synonym: positive selectable marker …   Glossary of Biotechnology

  • positive selectable marker — See: dominant selectable marker …   Glossary of Biotechnology

  • Marker gene — A marker gene is a gene used in molecular biology to determine if a nucleic acid sequence has been successfully inserted into an organism s DNA. There are two types of marker genes: a selectable marker and a marker for screening. Selectable… …   Wikipedia

  • marker gene — Gene that confers some readily detectable phenotype on cells carrying the gene, either in culture, or in transgenic or chimeric organisms. Gene could be an enzymic reporter gene, a selectable marker conferring antibiotic resistance, or a cell… …   Dictionary of molecular biology

  • Marker assisted selection — or marker aided selection (MAS) is a process whereby a marker (morphological, biochemical or one based on DNA/RNA variation) is used for indirect selection of a genetic determinant or determinants of a trait of interest (i.e. productivity,… …   Wikipedia

  • chimeric selectable marker gene — A gene that is constructed from parts of two or more different genes and allows the host cell to survive under conditions where it would otherwise die …   Glossary of Biotechnology

  • Scorable Marker — Als Scorable Marker wird in der Gentechnik ein Gen bezeichnet, das zusammen mit dem „gene of interest“, also dem eigentlich gewünschten Gen, in den veränderten Organismus eingebracht wird, um Individuen mit erfolgreicher Genveränderung erkennen… …   Deutsch Wikipedia

  • Delitto perfetto — is a genetic technique for in vivo site directed mutagenesis in yeast. This name is an Italian term for perfect deletion and is also an idiom for perfect murder. The name refers to the ability of the technique to create desired genetic changes… …   Wikipedia

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