Microsatellite enrichment
- Microsatellite enrichment
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Microsatellite enrichment is a method in molecular biology used for enriching the amount of microsatellite sequences in a DNA sample. This can be achieved by designing oligonucleotide probes that hybridize with the repeats in the microsatellites and then pull out the probe/microsatellite complexes from the solution.[1] This has been shown to be a cost effective method to sample the genetic diversity in non-model organisms.[2]
References
- ^ Kaukinen KH, Supernault KJ, and Miller KM (2004). "Enrichment of tetranucleotide microsatellite loci from invertebrate species". Journal of Shellfish Research 23 (2): 621.
- ^ Jennings, TN; Knaus, BJ, Mullins, TD, Haig, SM, Cronn, RC (2011 Jun 16). "Multiplexed microsatellite recovery using massively parallel sequencing.". Molecular ecology resources. PMID 21676207.
Categories:
- Molecular biology techniques
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