Nanodisc

A nanodisc is a synthetic model membrane system which assists in the study of membrane proteins. Nanodiscs are useful for membrane biology in the study of membrane proteins because they represent a more native environment than liposomes, detergent micelles or bicelles and allow the study of purified membrane proteins. Nanodiscs consist of a small portion of membrane that has been solubilized by the addition of two molecules of an amphipathic protein, dubbed the membrane scaffold protein (MSP). This protein wraps around the hydrophobic core of the lipids, effectively creating a soluble portion of membrane. When prepared properly, nanodiscs are uniform in size allow a native local environment to be maintained within a larger in vitro study.

Further reading

Some recent publications and reviews

Denisov, I.G., and Sligar, S.G., (2010) “Cytochromes P450 in Nanodiscs.” BBA - Proteins and Proteomics, doi: 10.1016/j.bbapap.2010.05.017

Ritchie, T.K., Grinkova, Y.V., Bayburt, T.H., Denisov, I.G., Zolnerciks, J.K., Atkins, W.M., and Sligar, S.G. (2009) “Reconstitution of Membrane Proteins in Phospholipid Bilayer Nanodiscs.” Methods, Enzymol. 464, 211-231. PMID: 19903557

Kijac, A., Shih, A., Nieuwkoop, A., Sligar, S. G., and Rienstra, C. (2009) “Lipid-Protein Correlations in Nanoscale Phospholipid Bilayers by Solid-State NMR.” Biochemistry, submitted. Bayburt, T. H., and Sligar, S. G. (2010) “Membrane Protein Assembly into Nanodiscs.” FEBS Letters 584, 1721-1727. PMID: 19836392

Morrissey, J. H., Pureza, V., Davis-Harrison, R. L., Sligar, S. G., Rienstra, C. M., Kijac, A. Z., Ohkubo, Y. Z., and Tajkhorshid, E. (2009) “Protein-Membrane Interactions: Blood Clotting on Nanoscale Bilayers.” J. Thrombosis and Haemostasis, 7, 169-172. PMID: 19630793

Shih, A.Y. Sligar, S. G., and Schulten, K. (2009) “Maturation of High-Density Lipoproteins.” J. Royal Society, Interface, 6, 863-871. PMID: 19570799 PMCID: PMC2805102

Das, A., Zhao, J., Schatz, G. C., Sligar, S. G., and Van Duyne, R. P. (2009) “Screening of Type I and II Drug Binding to Human Cytochrome P450-3A4 in Nanodiscs by Localized Surface Plasmon Resonance Spectroscopy.” Anal. Chem. 81, 3754-3759. PMID: 19364136

Shih, A.Y., Freddolino, P.L., Arkhipov, A., Sligar, S.G., and Schulten, K. (2008) “Molecular Modeling of the Structural Properties and Formation of High-Density Lipoprotein Particles.” Current Topics in Membranes 60, 313-342.

Goluch, E.D., Shaw, A. W., Sligar, S.G,, and Liu, C. (2008) “Microfluidic Patterning of Nanodisc Lipid Bilayers and Multiplexed Analysis of Protein Interaction.” Lab Chip 8, 1723-1728. PMID: 18813396

Zhao, J., Das, A., Schatz, G.C., Sligar, S.G., and VanDuyne, R. P. (2008) “Resonance Localized Surface Plasmon Spectroscoy: Sensing Substrate and Inhibitor Binding to Cytochrome P450.” J. Phys. Chem,112, 13084-13088. PMID: 16925400

Borch, J., Torta, F., Sligar, S. G., and Roepstorff, P. (2008) “Nanodiscs for Immobilization of Lipid Bilayers and Membrane Receptors: Kinetic Analysis of Cholera Toxin Binding to a Glycolipid Receptor.” Analytical Chemistry, 80, 6245-6252. PMID: 18616345

Morrissey, J. H., Pureza, V., Davis-Harrison, R. L., Sligar, S. G., Ohkubo, Y. Z. and Tajkhorshid, E. (2008) “Blood Clotting Reactions on Nanoscale Phospholipid Bilayers” Thrombosis Research, 122 (Supplement 1), S23-S26.

Marin, V. L., Bayburt, T. H., Sligar, S. G., and Mrksich, M. (2007) “Functional Assays of Membrane-Bound Proteins with SAMDI-TOF Mass Spectrometry.” Angew. Chem, Int. Ed 46, 8796-8798. PMID: 17943935

Kijac, A.Z., Li, Y., Sligar, S.G., and Rienstra, C.M. (2007) “Magic-Angle Spinning Solid-State NMR Spectroscopy of Nanodisc-Embedded Human CYP3A4.” Biochemistry 46, 13696-13703. PMID: 17985934

Shih, A.Y., Arkhipov, A., Freddolino, P.L., Sligar, S.G. and Schulten, K. (2007) “Assembly of Lipids and Proteins into Lipoprotein Particles.” J Phys Chem B, 111, 11095-11104. PMID: 17696388

Shih, A. Y., Freddolino, P. L., Sligar, S. G. and Schulten, K. (2007) “Disassembly of Nanodiscs with Cholate.” NanoLetters, 7, 1692-1696. PMID: 17503871

Bayburt, T.H., Leitz, A.J., Xie, G., Oprian, D.D., and Sligar, S.G. (2007) “Transducin Activation by Nanoscale Lipid Bilayers Containing One and Two Rhodopsins.” J. Biol. Chem., 282, 14875-14881. PMID: 17395586

Nath, A., Atkins, W. M., and Sligar, S. G. (2007) “Applications of Phospholipid Bilayer Nanodiscs in the Study of Membranes and Membrane Proteins.” Biochemistry 46, 2059-2069. PMID: 17263563

• Self-Assembly of Discoidal Phospholipid Bilayer Nanoparticles with Membrane Scaffold Proteins (ACS 10.1021/nl025623k) TH Bayburt, YV Grinkova & SG Sligar, 2002.

References

External links

• Nandisc Technology from the Stephen Sligar laboratory
• HDL and Nanodiscs an overview of nanodisc technology at UIUC
• Phospholipid Bilayer Nanodiscs A summary from the Atkins lab at the University of Washington
• Purchase the MSP The plasmid for the MSP is available from AddGene