Name= glucuronidase, beta
enzymes that separate glucuronic acidmolecules from other molecules by cutting glycosidic bonds. They are thus classified as glycoside hydrolases that cleave glucuronides.
eukaryotes, glucuronidase is located in lysosomes and plays an important role in recycling cellular components by cleaving glucuronide moieties from proteins. Glucuronidase exhibits both endo- glycosidaseand exo- glycosidaseactivities, meaning that it can cleave monosaccharides from the middle of a chain or from the end.
Preparations of beta-glucuronidase from the snail "
Helix pomatia" are commercially available and referred to by the trademark "Glusulase". These enzymes preparations are used to digest the ascusof the yeast" Saccharomyces cerevisiae" after sporulation.
E. coli" is among the few bacteria that can synthezise glucuronidase, and this trait is commonly used to identify the bacterium.
Sly syndromeis an inherited disease in which a genetic defect leads to a deficiency in glucuronidase. This results in numerous symptoms because of the accumulation of complex carbohydrates in many tissues and organs of the body.
Use as reporter gene
molecular biology, beta-glucuronidase is used as a reporter geneto monitor gene expression. Monitoring glucuronidase activity using a so-called GUS assayallows determination of the spatial and temporal expression of the gene being monitored. The beta-glucuronidase enzyme transforms certain substrates into colored or fluorescent products which can then be observed and measured.
*cite journal | author=George J.|title=Elevated serum beta-glucuronidase reflects hepatic lysosomal fragility following toxic liver injury in rats. |journal=Biochem Cell Biol. |volume=86|issue= 3 |pages= 235-243. |year= 2008 |pmid= 18523484 |doi= 10.1139/O08-038
* [http://www.reportergene.com/search/label/other%20reporters Updated research on reporter glucuronidase] and other reporters from Reportergene
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