Fluorescein isothiocyanate
- Fluorescein isothiocyanate
Chembox new
ImageFile=FITC-2D-skeletal.pngImageSize=
IUPACName=
OtherNames=FITC
Section1= Chembox Identifiers
CASNo=27072-45-3
PubChem=18730
SMILES=C1=CC2=C(C=C1N=C=S)C(=O) OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)O
MeSHName=Fluorescein-5-isothiocyanate
Section2= Chembox Properties
Formula=C21H11NO5S
MolarMass=389.382
Appearance=
Density=
MeltingPt=359.5 °C
BoilingPt=
Solubility=
Section3= Chembox Hazards
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Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. :A succinimidyl-ester functional group attached to the fluorescein core, creating NHS-fluorescein, forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. Newer derivatives of fluorescein such as Alexa 488 and DyLight 488, have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.
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fluorescein isothiocyanate — A fluorochrome dye frequently coupled to antibodies that are used to locate and identify specific antigens. * * * (FITC) a fluorescein derivative that can be conjugated to proteins, particularly antibodies, and is used as a fluorescent label in… … Medical dictionary
fluorescein isothiocyanate — noun a fluorochrome commonly conjugated with antibodies for use in indirect immunofluorescence • Syn: ↑fluorescein isocyanate • Hypernyms: ↑fluorochrome … Useful english dictionary
Isothiocyanate de fluorescéine — Général Nom IUPAC 3 ,6 dihydroxy 5(ou 6) isothiocyanatospiro[isobenzofuranne 1(3H),9 [9H]xanthène] 3 one … Wikipédia en Français
Fluorescein — Chembox new ImageFile = Fluoresceine.svg ImageSize = ImageFile2 = Fluorescein sample.jpg IUPACName = OtherNames = Fluorescein, resorcinolphthalein, C.I. 45350, solvent yellow 94, D C yellow no. 7, angiofluor, Japan yellow 201, soap yellow,… … Wikipedia
fluorescein isocyanate — noun a fluorochrome commonly conjugated with antibodies for use in indirect immunofluorescence • Syn: ↑fluorescein isothiocyanate • Hypernyms: ↑fluorochrome … Useful english dictionary
fluorescein — Fluorophore commonly used in microscopy. Fluorescein di acetate can be used as a vital stain, or can be conjugated to proteins (particularly antibodies) using isothiocyanate. Excitation is at 365nm, and the emitted light is green yellow (450… … Dictionary of molecular biology
FITC — • fluorescein isothiocyanate … Dictionary of medical acronyms & abbreviations
FITC — (= fluorescein isothiocyanate) FITC is used as a reagent to conjugate fluorescein to protein. FITC labelled antibodies are extensively used for fluorescence microscopy: the fluorophore illuminated with UV emits a yellow green light … Dictionary of molecular biology
FITC — Fluorescein IsoThioCyanate (Medical » Human Genome) * Federación Independiente de Trabajadores de Crédito (International » Spanish) … Abbreviations dictionary
FIT — • fluorescein isothiocyanate; • Fracture Intervention Trial; • fusion inferred threshold … Dictionary of medical acronyms & abbreviations
