Fluorescein isothiocyanate

Fluorescein isothiocyanate

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Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. :A succinimidyl-ester functional group attached to the fluorescein core, creating NHS-fluorescein, forms another common amine reactive derivative that has much greater specificity toward primary amines in the presence of other nucleophiles. Newer derivatives of fluorescein such as Alexa 488 and DyLight 488, have been tailored for various chemical and biological applications where greater photostability, higher fluorescence intensity, or different attachment groups are needed.

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  • fluorescein isocyanate — noun a fluorochrome commonly conjugated with antibodies for use in indirect immunofluorescence • Syn: ↑fluorescein isothiocyanate • Hypernyms: ↑fluorochrome …   Useful english dictionary

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  • FITC — • fluorescein isothiocyanate …   Dictionary of medical acronyms & abbreviations

  • FITC — (= fluorescein isothiocyanate) FITC is used as a reagent to conjugate fluorescein to protein. FITC labelled antibodies are extensively used for fluorescence microscopy: the fluorophore illuminated with UV emits a yellow green light …   Dictionary of molecular biology

  • FITC — Fluorescein IsoThioCyanate (Medical » Human Genome) * Federación Independiente de Trabajadores de Crédito (International » Spanish) …   Abbreviations dictionary

  • FIT — • fluorescein isothiocyanate; • Fracture Intervention Trial; • fusion inferred threshold …   Dictionary of medical acronyms & abbreviations

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