Kinetic capillary electrophoresis

Kinetic capillary electrophoresis

Kinetic capillary electrophoresis or KCE is capillary electrophoresis of molecules that interact during electrophoresis. KCE was introduced by Professor Sergey Krylov and his research group at York University, Toronto, Canada [KCE] . It serves as a conceptual platform for development of homogeneous chemical affinity methods for studies of molecular interactions (measurements of binding and rate constants) and affinity purification (purification of known molecules and search for unknown molecules). Different KCE methods are designed by varying initial and boundary conditions – the way interacting molecules enter and exit the capillary. Several KCE methods were described: non-equilibrium capillary electrophoresis of the equilibrium mixtures (NECEEM) [NECEEM] , sweeping capillary electrophoresis (SweepCE) [SweepCE] , plug-plug KCE (ppKCE) [ppKCE] .

References

1. Petrov, A.; Okhonin, V.; Berezovski, M.; Krylov, S.N. "Kinetic capillary electrophoresis (KCE): a conceptual platform for kinetic homogeneous affinity methods." Journal of the American Chemical Society 2005, 127, 17104-17110. [http://www.ncbi.nlm.nih.gov/pubmed/16316258?ordinalpos=11&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum, PubMed]
2. Berezovski, M.; Krylov, S.N. "Nonequilibrium capillary electrophoresis of equilibrium mixtures - a single experiment reveals equilibrium and kinetic parameters of protein-DNA interactions." Journal of the American Chemical Society 2002, 124, 13674-13675. [http://www.ncbi.nlm.nih.gov/pubmed/12431087?ordinalpos=21&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum, PubMed]
3. Okhonin, V.; Berezovski, M.; Krylov, S.N. "Sweeping capillary electrophoresis – a non-stopped-flow method for measuring bimolecular rate constant of complex formation between protein and DNA." Journal of the American Chemical Society 2004, 126, 7166-7167. [http://www.ncbi.nlm.nih.gov/pubmed/15186140?ordinalpos=17&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum, PubMed]
4. Okhonin, V.; Petrov, A.; Berezovski, M.; Krylov, S.N. "Plug-plug kinetic capillary electrophoresis: a method for direct determination of rate constants of complex formation and dissociation." Analytical Chemistry 2006, 78, 4803-4810. [http://www.ncbi.nlm.nih.gov/pubmed/16841898?ordinalpos=6&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum, PubMed]


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