NPEPPS

Aminopeptidase puromycin sensitive
Identifiers
Symbols	NPEPPS; AAP-S; MP100; PSA
External IDs	OMIM: 606793 MGI: 1101358 HomoloGene: 36199 GeneCards: NPEPPS Gene
EC number	3.4.11.14
Gene Ontology Molecular function • aminopeptidase activity • protein binding • peptidase activity • metallopeptidase activity • zinc ion binding • metal ion binding Cellular component • nucleus • cytoplasm • cytosol Biological process • proteolysis Sources: Amigo / QuickGO
Orthologs
Species	Human	Mouse
Entrez	9520	19155
Ensembl	ENSG00000141279	ENSMUSG00000001441
UniProt	P55786	Q3UZE0
RefSeq (mRNA)	XM_001128588	NM_008942.2
RefSeq (protein)	XP_001128588	NP_032968.2
Location (UCSC)	Chr 17: 45.6 – 45.7 Mb	Chr 11: 97.07 – 97.14 Mb
PubMed search	[1]	[2]

Puromycin-sensitive aminopeptidase is an enzyme that in humans is encoded by the NPEPPS gene.^[1][2][3]

This gene encodes the puromycin-sensitive aminopeptidase, a zinc metallopeptidase which hydrolyzes amino acids from the N-terminus of its substrate. The protein has been localized to both the cytoplasm and to cellular membranes. This enzyme degrades enkaphalins in the brain, and studies in mouse suggest that it is involved in proteolytic events regulating the cell cycle.^[3]

References

1. ^ Tobler AR, Constam DB, Schmitt-Graff A, Malipiero U, Schlapbach R, Fontana A (Apr 1997). "Cloning of the human puromycin-sensitive aminopeptidase and evidence for expression in neurons". J Neurochem 68 (3): 889–97. doi:10.1046/j.1471-4159.1997.68030889.x. PMID 9048733. 
2. ^ Thompson MW, Tobler A, Fontana A, Hersh LB (Jun 1999). "Cloning and analysis of the gene for the human puromycin-sensitive aminopeptidase". Biochem Biophys Res Commun 258 (2): 234–40. doi:10.1006/bbrc.1999.0604. PMID 10329370. 
3. ^ ^{a b} "Entrez Gene: NPEPPS aminopeptidase puromycin sensitive". http://www.ncbi.nlm.nih.gov/sites/entrez?Db=gene&Cmd=ShowDetailView&TermToSearch=9520. 

Further reading

• Ewing RM, Chu P, Elisma F et al. (2007). "Large-scale mapping of human protein–protein interactions by mass spectrometry". Mol. Syst. Biol. 3 (1): 89. doi:10.1038/msb4100134. PMC 1847948. PMID 17353931. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=1847948. 
• Gerhard DS, Wagner L, Feingold EA et al. (2004). "The Status, Quality, and Expansion of the NIH Full-Length cDNA Project: The Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=528928. 
• Thompson MW, Hersh LB (2005). "Analysis of conserved residues of the human puromycin-sensitive aminopeptidase". Peptides 24 (9): 1359–65. doi:10.1016/j.peptides.2003.07.012. PMID 14706550. 
• Ota T, Suzuki Y, Nishikawa T et al. (2004). "Complete sequencing and characterization of 21,243 full-length human cDNAs". Nat. Genet. 36 (1): 40–5. doi:10.1038/ng1285. PMID 14702039. 
• Strausberg RL, Feingold EA, Grouse LH et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932. http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pmcentrez&artid=139241. 
• Bauer WO, Nanda I, Beck G et al. (2001). "Human puromycin-sensitive aminopeptidase: cloning of 3' UTR, evidence for a polymorphism at a.a. 140 and refined chromosomal localization to 17q21". Cytogenet. Cell Genet. 92 (3–4): 221–4. doi:10.1159/000056907. PMID 11435692. 
• de Gandarias JM, Irazusta J, Gil J et al. (1999). "Ontogeny of puromycin-sensitive and insensitive aminopeptidase activities in several subcellular fractions of the rat brain". Brain Res. Bull. 50 (4): 283–90. doi:10.1016/S0361-9230(99)00189-6. PMID 10582526. 
• Huber G, Thompson A, Grüninger F et al. (1999). "cDNA cloning and molecular characterization of human brain metalloprotease MP100: a beta-secretase candidate?". J. Neurochem. 72 (3): 1215–23. doi:10.1046/j.1471-4159.1999.0721215.x. PMID 10037494. 
• Bonaldo MF, Lennon G, Soares MB (1997). "Normalization and subtraction: two approaches to facilitate gene discovery". Genome Res. 6 (9): 791–806. doi:10.1101/gr.6.9.791. PMID 8889548. 
• Cussenot O, Berthon P, Cochand-Priollet B et al. (1994). "Immunocytochemical comparison of cultured normal epithelial prostatic cells with prostatic tissue sections". Exp. Cell Res. 214 (1): 83–92. doi:10.1006/excr.1994.1236. PMID 8082751. 
• Constam DB, Tobler AR, Rensing-Ehl A et al. (1995). "Puromycin-sensitive aminopeptidase. Sequence analysis, expression, and functional characterization". J. Biol. Chem. 270 (45): 26931–9. doi:10.1074/jbc.270.45.26931. PMID 7592939. 
• McLellan S, Dyer SH, Rodriguez G, Hersh LB (1988). "Studies on the tissue distribution of the puromycin-sensitive enkephalin-degrading aminopeptidases". J. Neurochem. 51 (5): 1552–9. doi:10.1111/j.1471-4159.1988.tb01124.x. PMID 3171591.