Lucifer yellow

Lucifer yellow

Lucifer yellow is a fluorescent dye used in cell biology. For common usage it is compounded with carbohydrazide (CH) and prepared as a Lithium salt. The CH group allows it to be covalently linked to surrounding biomolecules during aldehyde fixation. The key property of Lucifer yellow is that it can readily visualized in both living and fixed cells using a fluorescence microscope.

Lucifer yellow was engineered by Walter W. Stewart at NIH and patented in 1978. [ [http://www.freepatentsonline.com/4473693.html Patent description] ]

Alternate preparations

Other cations such as ammonium or potassium can be used when lithium is undesirable, but the the resulting salts are less soluble in water.

Lucifer yellow can also be compounded as a vinyl sulfone, with ethylenediamine, or with cadaverine.

References

* [http://probes.invitrogen.com/media/pis/mp00453.pdf Invitrogen's manual for Lucifer yellow]
* [http://omlc.ogi.edu/spectra/PhotochemCAD/html/luciferyellowCH.html Molecular structure and spectra of Lucifer yellow CH (lithium salt)]


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