Ehrlich's reagent

Ehrlich's reagent

Ehrlich's reagent (p-dimethyl-aminobenzaldehyde dissolved in 20% HCL). It is used for detection of urobilinogen in fresh cool urine.

If urine sample is left to oxidize in air to form urobilin the reagent will not detect the urobilinogen.

By adding few drops of reagent to 3 ml of urine in a test tube one can see a change of color from above, to dark pink or red. The degree of color change is proportional to the amount of urobilinogen in the urine sample.

Ehrlich's reagent is a strong electrophile which reacts with the electron-rich ά-carbon of indole rings to form a blue-coloured adduct. it can be used to detect the presence of indole alkaloids. Not all indole alkaloids give a coloured adduct as result of steric hinderance which does not allow the reaction to proceed


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