Exon trapping

Exon trapping

Exon trapping is a molecular biology technique to identify potential exons in a fragment of eukaryote DNA of unknown intron-exon structure. This is done to determine if the fragment is part of an expressed gene.

The genomic fragment is inserted into the intron of a 'splicing vector' consisting of a known exon - intron - exon sequence of DNA. If the fragment does not contain exons (i.e., consists solely of intron DNA), it will be spliced out together with the vector's original intron. On the other hand, if exons are contained, they will be part of the mature mRNA after transcription (with all intron material removed). The presence of 'trapped exons' can be detected by an increase in size of the mRNA.


Wikimedia Foundation. 2010.

Игры ⚽ Поможем написать реферат

Look at other dictionaries:

  • Exon Trapping — Übersicht über das Exon Trapping. Vom dargestellten Plasmid wird eine RNA transkribiert die ein Intron (blaue Linie) flankiert von zwei Exons (gelbe Kästen) enthält. Durch Splicing wird das Intron entfernt. Befindet sich nun eine beliebige… …   Deutsch Wikipedia

  • exon trapping — Technique for identifying regions of a genomic DNA fragment that are part of an expressed gene. The genomic sequence is cloned into an intron, flanked by two exons, in a specialized exon trapping vector, and the construct expressed through a… …   Dictionary of molecular biology

  • Exon — An exon is a nucleic acid sequence that is represented in the mature form of an RNA molecule after a) portions of a precursor RNA, introns, have been removed by cis splicing or b) two or more precursor RNA molecules have been ligated by trans… …   Wikipedia

  • Exon — Schematische Darstellung von Exons und Introns auf der Ebene der DNA, prä mRNA und mRNA. Als Exon (von engl. expressed region) wird der Teil eines eukaryotischen Gens bezeichnet, der nach dem Spleißen (Splicing) erhalten bleibt. Dem gegenüber… …   Deutsch Wikipedia

  • Gene trapping — is a high throughput approach that is used to introduce insertional mutations across the mammalian genome. It is performed with gene trap vectors whose principal element is a gene trapping cassette consisting of a promoterless reporter gene… …   Wikipedia

  • Exons — Schematische Darstellung von Exons und Introns auf der Ebene der DNA, prä mRNA und mRNA. Ein Exon (von engl. expressed region) ist der Teil eines eukaryotischen Gens, der nach dem Spleißen (Splicing) erhalten bleibt und im Zuge der Protein… …   Deutsch Wikipedia

  • Genetic screen — This article is about a method to identify the functions of genes. For screening or testing for genetic diseases, see genetic testing. A genetic screen (often known as a phenotypic screen, or shortened to screen) is a procedure or test to… …   Wikipedia

  • ATP5O — ATP synthase, H+ transporting, mitochondrial F1 complex, O subunit (oligomycin sensitivity conferring protein), also known as ATP5O, is a human gene.cite web | title = Entrez Gene: ATP5O ATP synthase, H+ transporting, mitochondrial F1 complex, O… …   Wikipedia

  • Molecular Inversion Probe — (MIP)[1] belongs to the class of Capture by Circularization molecular techniques [1] for performing genomic partitioning, a process through which one captures and enriches specific regions of the genome[2]. Probes used in this technique are… …   Wikipedia

  • Gene targeting — ist eine genetische Technik, die die Rekombination ausnutzt, um ein endogenes Gen zu verändern. Die Methode kann verwendet werden, um ein Gen auszulöschen, Exons zu entfernen und Punktmutationen durchzuführen. Gene targeting kann permanent sein… …   Deutsch Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”