SraH RNA

SraH (also known as RyhA) is a is a small non-coding RNA (ncRNA). It is the functional product of a gene which is not translated into protein.

This non-coding RNA was discovered in the bacteria "Escherichia coli" during a large scale computational screen for transcription signals and genomic features of known small RNA-encoding genescite journal | author = Argaman L, Hershberg R, Vogel J, "et al" | title = Novel small RNA-encoding genes in the intergenic regions of Escherichia coli | journal = Curr. Biol. | volume = 11 | issue = 12 | pages = 941–50 | year = 2001 | pmid = 11448770 | doi = | issn = ] . During this screen 14 novel ncRNA genes were identified. It appeas to be widely conserved amongst the

The expression of SraH was experimentally confirmed by Northern blotting. Its expression is highly abundant in stationary growth phase but low levels of expression can still be detected in exponentially growing cells .

Although SraH is initally transcribed as a transcript of ~120 nucleotides. This precursor is instable and is processed into an abundant fragment ~58 nucleotides which represents the 3' end of the initial trascript. The stablity and abundance of the shorter 3' transcript is confirmed in both Northern blotting and deep sequencing analysis. cite journal |author=Zhang A, Wassarman KM, Rosenow C, Tjaden BC, Storz G, Gottesman S |title=Global analysis of small RNA and mRNA targets of Hfq |journal=Molecular microbiology |volume=50 |issue=4 |pages=1111–24 |year=2003 |month=November |pmid=14622403 |doi= |url=http://www.blackwell-synergy.com/openurl?genre=article&sid=nlm:pubmed&issn=0950-382X&date=2003&volume=50&issue=4&spage=1111 ] cite journal |author=Sittka A, Lucchini S, Papenfort K, "et al" |title=Deep sequencing analysis of small noncoding RNA and mRNA targets of the global post-transcriptional regulator, Hfq |journal=PLoS genetics |volume=4 |issue=8 |pages=e1000163 |year=2008 |pmid=18725932 |pmc=2515195 |doi=10.1371/journal.pgen.1000163 |url=]

The function of SraH is not known but it is likely to act as an antisense regulator of some bacterial genes. Its has been shown to strongly bind the global post-transcriptional regulator protein Hfq.

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