Small nucleolar RNA R66

Small nucleolar RNA R66 (also known as snoR66) is a non-coding RNA (ncRNA) molecule which functions in the modification of other small nuclear RNAs (snRNAs). This type of modifiying RNA is usually located in the nucleolus of the eukaryotic cell which is a major site of snRNA biogenesis. It is known as a small nucleolar RNA (snoRNA) and also often referred to as a guide RNA.

snoR66 belongs to the C/D box class of snoRNAs which contain the conserved sequence motifs known as the C box (UGAUGA) and the D box (CUGA). Most of the members of the box C/D family function in directing site-specific 2'-O-methylation of substrate RNAs [cite journal | last = Galardi | first = S | coauthors = Fatica A, Bachi A, Scaloni A, Presutti C, Bozzoni I | year = 2002 | title = Purified box C/D snoRNPs are able to reproduce site-specific 2'-O-methylation of target RNA in vitro | journal = Mol Cell Biol | volume = 22 | pages = 6663–6668 | pmid = 12215523 | doi = 10.1128/MCB.22.19.6663-6668.2002] .

snoR66 was identified by a computational screening of the rice "Oryza sativa" genome and is proposed to acts as a methylation guide for 18S ribosomal RNA in plants cite journal | author = Chen CL, Liang D, Zhou H, Zhuo M, Chen YQ, Qu LH | title = The high diversity of snoRNAs in plants: identification and comparative study of 120 snoRNA genes from Oryza sativa | journal = Nucleic Acids Res. | volume = 31 | issue = 10 | pages = 2601–13 | year = 2003 | pmid = 12736310 | doi = | issn = ] . Rice snoR66 has also been alternatively named snoZ269. Its should also not be confused with the snoRNA identified in yeast ("Saccharomyces cerevisiae") and called snR66 cite journal | author = Lowe TM, Eddy SR | title = A computational screen for methylation guide snoRNAs in yeast | journal = Science | volume = 283 | issue = 5405 | pages = 1168–71 | year = 1999 | pmid = 10024243 | doi = | issn = ] .

References

External links

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* [http://bioinf.scri.sari.ac.uk/cgi-bin/plant_snorna/home plant snoRNA database]
* [http://people.biochem.umass.edu/fournierlab/snornadb/main.php yeast snoRNA database]