A fed-batch is a biotechnological batch process which is based on feeding of a growth limiting nutrient substrate to a culture. The fed-batch strategy is typically used in bio-industrial processes to reach a high cell density in the bioreactor. Mostly the feed solution is highly concentrated to avoid dilution of the bioreactor.The controlled addition of the nutrient directly affects the growth rate of the culture and allows to avoid overflow metabolism (formation of side metabolites, such as acetate for "Escherichia coli", lactic acid in cell cultures, ethanol in "Saccharomyces cerevisiae"), oxygen limitation (anaerobiosis).In most cases the growth-limiting nutrient is glucose which is fed to the culture as a highly concentrated glucose syrup (600-850 g/l).

Why is the fed-batch principle used?- Substrate limitation offers the possibility to control the reaction rates to avoid technological limitations connected to the cooling of the reactor and oxygen transfer.- Substrate limitation also allows the metabolic control, to avoid osmotic effects, catabolite repression and overflow metabolism of side products.

Different strategies can be used to control the growth in a fed-batch process:


* Hewitt CJ, Nienow AW: The scale-up of microbial batch and fed-batch fermentation processes. Adv Appl Microbiol 2007, 62:105-135.
* Wlaschin KF, Hu WS: Fedbatch culture and dynamic nutrient feeding. Cell Culture Engineering 2006, 101:43-74.
* Shiloach J, Fass R: Growing E. coli to high cell density--a historical perspective on method development. Biotechnol Adv 2005, 23:345-357.
* Panda AK: Bioprocessing of therapeutic proteins from the inclusion bodies of Escherichia coli. Adv Biochem Eng Biotechnol 2003, 85:43-93.
* Liden G: Understanding the bioreactor. Bioprocess and Biosystems Engineering 2002, 24:273-279.
* Neubauer P, Winter J: Expression and fermentation strategies for recombinant protein production in Escherichia coli. In: Merten OW et al. (Eds). Recombinant Protein Production with prokaryotic and eukaryotic cells. A comparative view on host physiology. 2001, Kluwer Academic Publisher, Dortrecht, The Netherlands. pp. 196-260.
* Balbas P: Understanding the art of producing protein and nonprotein molecules in Escherichia coli. Molecular Biotechnology 2001, 19:251-267.
* Lee J, Lee SY, Park S, Middelberg

* Zhang J, Greasham R: Chemically defined media for commercial fermentations. Applied Microbiology and Biotechnology 1999, 51:407-421.
* Lee SY: High cell-density culture of Escherichia coli. Trends Biotechnol 1996, 14:98-105.
* Mendozavega O, Sabatie J, Brown SW: Industrial-Production of Heterologous Proteins by Fed-Batch Cultures of the Yeast Saccharomyces-Cerevisiae. Fems Microbiology Reviews 1994, 15:369-410.
* Yee L, Blanch HW: Recombinant protein expression in high cell density fed-batch cultures of Escherichia coli. Biotechnology (N Y ) 1992, 10:1550-1556.
* Riesenberg D: High-cell-density cultivation of Escherichia coli. Curr Opin Biotechnol 1991, 2:380-384.

Wikimedia Foundation. 2010.

Игры ⚽ Поможем написать курсовую

Look at other dictionaries:

  • Fed-Batch-Prozess — Schema Fed Batch Prozess (englisch fed „gefüttert“ und batch „Stapel“)auch Zulauf Verfahren ist ein in der Verfahrenstechnik etablierter Begriff. Man bezeichnet damit Prozesse, die als „Stapel“, das heißt nacheinander, abgearbeitet werden… …   Deutsch Wikipedia

  • fed-batch fermentation — Culture of cells or micro organisms where nutrients are added periodically to the bioreactor …   Glossary of Biotechnology

  • Batch-Kultur — (Syn. Satz , Ansatz , statische oder diskontinuierliche Kultur) bezeichnet ein Anzuchtverfahren für Mikroorganismen. Dabei werden die Mikroorganismen in ein Kulturmedium eingebracht. Das Medium bleibt während der gesamten Anzucht das Gleiche,… …   Deutsch Wikipedia

  • batch processing — A method of processing data, using a computer, in which the programs to be executed are collected together into groups, or batches, for processing. All the information needed to execute the programs is loaded into the computer at the start so… …   Big dictionary of business and management

  • Batch Process —   A process for carrying out a reaction in which the reactants are fed in discrete and successive charges …   Energy terms

  • Mass balance — A mass balance (also called a material balance) is an application of conservation of mass to the analysis of physical systems. By accounting for material entering and leaving a system, mass flows can be identified which might have been unknown,… …   Wikipedia

  • Bioréacteur — Principe du bioréacteur Un bioréacteur, appelé également fermenteur ou propagateur, est un appareil dans lequel on multiplie des micro organismes (levures, bactéries, champignons microscopiques, algues, cellules animales et végétales) pour la… …   Wikipédia en Français

  • Bioreaktor — Viele Produkte der roten Biotechnologie werden in Bioreaktoren produziert, wie in dieser Anlage zur Herstellung von Vakzinen …   Deutsch Wikipedia

  • Bioréacteur jetable — Un bioréacteur jetable consiste en une chambre de culture stérile conçue en matériel polymérique, approuvé par la Food and Drug Administration (FDA) [1]. Après avoir rempli le bioréacteur de milieu de culture et d’inoculum avec l’organisme de… …   Wikipédia en Français

  • Reacteur (chimie) — Réacteur (chimie) Pour les articles homonymes, voir Réacteur. En chimie, un réacteur est une enceinte ou récipient apte à la réalisation et l optimisation de réactions chimiques et généralement de procédés de transformation de la matière (génie… …   Wikipédia en Français

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”