Hyperchromic effect

Hyperchromic effect

The hyperchromic effect is the striking increase in absorbance of DNA caused by the denaturation of the double-stranded DNA molecule. The two strands of DNA are bound together by the hydrogen bonds between the complementary bases. The hydrogen bond limits the resonance of the aromatic ring so the absorbance of the sample is limited as well. When the DNA double helix is treated with denatured agents, the interaction force holding the double helical structure is disrupted. The double helix then separates into two single strands which are in the random coiled conformation. At this time, the base-base interaction will be reduced, increasing the UV absorbance of DNA solution because many bases are in free form and do not form hydrogen bonds with complementary bases. As a result, the absorbance for single-stranded DNA will be 40% higher than that for double stranded DNA at the same concentration.


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  • hyperchromic effect — hyperchromic effect. См. гиперхромизм. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • hyperchromic effect — hiperchrominis efektas statusas T sritis chemija apibrėžtis Tirpalo ekstinkcijos padidėjimas pakitus ištirpusios medžiagos struktūrai. atitikmenys: angl. hyperchromic effect rus. гиперхромный эффект …   Chemijos terminų aiškinamasis žodynas

  • hyperchromic — 1. SYN: hyperchromatic (1). 2. Denoting increased light absorption. 3. Denoting more highly colored than normal. 4. Describing erythrocytes that contain, or appear to contain, more hemoglobin than normal. * * * hy·per·chro·mic krō mik adj 1) of,… …   Medical dictionary

  • effect — The result or consequence of an action. [L. efficio, pp. effectus, to accomplish, fr. facio, to do] abscopal e. a reaction produced following irradiation but occurring outside the zone of actual radiation absorption. additive e. an e. wherein two …   Medical dictionary

  • гиперхромизм — hyperchromic effect, hyperchromicity гиперхромный эффект, гиперхромизм. Повышение оптической плотности раствора нуклеиновых кислот (особенно двухцепочечной ДНК) в результате их денатурации нагреванием, действием щелочных растворов и т.п.; Г.э.… …   Молекулярная биология и генетика. Толковый словарь.

  • List of effects — This is a list of names for observable phenonema that contain the word effect, amplified by reference(s) to their respective fields of study. #*3D audio effect (audio effects)A*Accelerator effect (economics) *Accordion effect (physics) (waves)… …   Wikipedia

  • гиперхромный сдвиг г эффект гиперхромизм — гиперхромный сдвиг, г. эффект, гиперхромизм * гіперхромны зрух, г. эфект, гіперхрамізм * hyperchromic effect or h. shift or hyperchromiсity рост оптической плотности за счет увеличения адсорбции УФ в растворе двунитчатой ДНК по мере повышения… …   Генетика. Энциклопедический словарь

  • Deoxyribonuclease — Crystals of DNase protein. A deoxyribonuclease (DNase, for short) is any enzyme that catalyzes the hydrolytic cleavage of phosphodiester linkages in the DNA backbone. Thus, deoxyribonucleases are one type of nuclease. A wide variety of… …   Wikipedia

  • hyperchromicity — hyperchromicity. = hyperchromic effect (см.). (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • hiperchrominis efektas — statusas T sritis chemija apibrėžtis Tirpalo ekstinkcijos padidėjimas pakitus ištirpusios medžiagos struktūrai. atitikmenys: angl. hyperchromic effect rus. гиперхромный эффект …   Chemijos terminų aiškinamasis žodynas

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