HIS3

HIS3

The HIS3 gene, found in the "Saccharomyces cerevisiae" yeast, encodes a protein called Imidazoleglycerol-phosphate dehydratase which catalyses the sixth step in histidine biosynthesis. [ [http://db.yeastgenome.org/cgi-bin/locus.pl?locus=his3 HIS3/YOR202W Summary ] ] It is analogous to hisB in "Escherichia coli".

Exploits

Mutations in "Escherichia coli"'s analogous gene, hisB allows researchers to select only those individuals expressing the HIS3 gene included on a plasmid. The HIS3 gene is coupled to a certain promoter which can only be activated by successful binding of the relevant transcription factors. This is used in certain methods of bacterial two-hybrid screening to allow the survival of "E. coli" in which a desired protein-DNA or protein-protein interaction is taking place (Joung et al., 2000)

References

*Joung, J. K., E. I. Ramm and C. O. Pabo (2000). "A bacterial two-hybrid selection system for studying protein-DNA and protein-protein interactions." Proc Natl Acad Sci U S A 97(13): 7382-7.


Wikimedia Foundation. 2010.

Игры ⚽ Поможем написать курсовую

Look at other dictionaries:

  • Two-hybrid screening — Overview of two hybrid assay, checking for interactions between two proteins, called here Bait and Prey . A. Gal4 transcription factor gene produces two domain protein ( BD and AD ) which is essential for transcription of the reporter gene ( LacZ …   Wikipedia

  • 3-Amino-1,2,4-triazole — chembox new Reference = [ [http://pmep.cce.cornell.edu/profiles/extoxnet/24d captan/amitrole ext.html EXTOXNET] Herbicide fact sheet for amitrole] ImageFile = Aminotriazole.png ImageSize = 150px IUPACName = 3 Amino 1,2,4 triazole OtherNames = 1,2 …   Wikipedia

  • Cromosoma artificial de levadura — El cromosoma artificial de levaduras o YAC (Yeast artificial chromosome) forma parte de los vectores de clonación de alta capacidad siendo, de hecho, el de mayor capacidad (200 kb 3.000 kb). Fueron descritos por primera vez en 1983 por Murray y… …   Wikipedia Español

  • Histidine — NatOrganicBox name=2 amino 3 (3H imidazol 4 yl)propanoic acid synonyms=Imidazole alanine PubChem = 773 CAS = 71 00 1 SMILES = N [C@@H] (Cc1 [nH] cnc1)C(O)=O C=6 | H=9 | N=3 | O=2 mass=155.16 g/molHistidine (abbreviated as His or H) [cite web |… …   Wikipedia

  • Postal Reorganization Act — The Postal Reorganization Act of 1970 abolished the United States Post Office Department, a part of the cabinet, and created the United States Postal Service, a corporation like independent agency with an official monopoly on the delivery of mail …   Wikipedia

  • Alexander Chee — Alexander Chee, gay American fiction writer, poet, journalist and reviewer.Born in Rhode Island, he spent his childhood in South Korea, Kuai, Truk, Guam and Maine. He attended Wesleyan University and the Iowa Writers Workshop. His short fiction… …   Wikipedia

  • HisB — The hisB gene, found in Escherichia coli encodes a protein involved in catalysis of histidine biosynthesis (Joung et al. , 2000). Analogous genes in other species HIS3 Saccharomyces cerevisiae References *Joung, J. K., E. I. Ramm and C. O. Pabo… …   Wikipedia

  • HIS-selective medium — is a type cell culture medium that lacks the amino acid histidine. It can be used with bacteria reliant on the expression of a gene encoding proteins involved in histidine expression in order to survive (Joung et al. , 2000). Only bacteria… …   Wikipedia

  • Tonhöhenklasse — ist ein Begriff aus der mathematischen Musiktheorie, die vor allem in Amerika verbreitet ist. Dort lautet sein begriffliches Äquivalent „pitch class“. Hintergrund Die menschliche Wahrnehmung von Tonhöhen ist periodisch. Tonhöhen, die eine volle… …   Deutsch Wikipedia

Share the article and excerpts

Direct link
Do a right-click on the link above
and select “Copy Link”