Sequence-tagged site

Sequence-tagged site

A sequence-tagged site (or STS) is a short (200 to 500 base pair) DNA sequence that has a single occurrence in the genome and whose location and base sequence are known.

STSs can be easily detected by the polymerase chain reaction (PCR) using specific primers. For this reason, they are used as genetic markers, which are useful for localizing and orienting the mapping and sequence data reported from many different laboratories. They serve as landmarks on the developing physical map of a genome.

When STS loci contain DNA length polymorphisms (e.g. simple sequence length polymorphisms, SSLPs), they become valuable genetic markers.

They are used in shotgun sequencing, specifically to aid sequence assembly.


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  • sequence tagged site — sequence tagged site. = STS (см.). (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • sequence tagged site — sequence tagged site. См. ДНК маркирующий сайт. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • sequence-tagged site — Abbreviation: STS Short unique DNA sequence (200 500 bp long) that can be amplified by PCR and is thus tagged to the site on the chromosome from which it was amplified …   Glossary of Biotechnology

  • Sequence tagged site (STS) — A short (200 to 500 base pair) DNA sequence that occurs but once in the genome and whose location and base sequence are known. STSs are detectable by polymerase chain reaction (PCR), are useful for localizing and orienting the mapping and… …   Medical dictionary

  • sequence-tagged site — (STS) a short length (200–500 base pairs) of DNA that has a single occurrence in the genome and can be detected and amplified by the polymerase chain reaction; useful in physical mapping of the genome …   Medical dictionary

  • STS (sequence-tagged site) map — a physical map indicating the relative order of and distance between sequence tagged sites (q.v.) within a DNA region …   Medical dictionary

  • sequence characterized amplified region — Abbreviation: SCAR A molecular marker obtained by the conversion to a sequence tagged site of a single random amplified polymorphic DNA product …   Glossary of Biotechnology

  • site — A place or location or locus. SYN: situs. [L. situs] acceptor s. the ribosomal binding s. for the aminoacyl tRNA during protein synthesis. acceptor splicing s. SYN: right splicing junction. active s. that portion of an …   Medical dictionary

  • EST (expressed sequence tag) — A unique stretch of DNA within a coding region of a gene that is useful for identifying full length genes and serves as a landmark for mapping. An EST is a sequence tagged site (STS) derived from cDNA. An STS is a short segment of DNA which… …   Medical dictionary

  • Expressed sequence tag (EST) — A unique stretch of DNA within a coding region of a gene that is useful for identifying full length genes and serves as a landmark for mapping. An EST is a sequence tagged site (STS) derived from cDNA. An STS is a short segment of DNA which… …   Medical dictionary

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