Bromodeoxyuridine

Bromodeoxyuridine

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ImageFile=Bromodeoxyuridine.svg
ImageSize=
IUPACName=
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Section1= Chembox Identifiers
CASNo=59-14-3
PubChem=6035
SMILES=C1C(C(OC1N2C=C(C(=O)NC2=O)Br)CO)O
MeSHName=Bromodeoxyuridine
Section2= Chembox Properties
Formula=C9H11BrN2O5
MolarMass=307.098
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Section3= Chembox Hazards
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Bromodeoxyuridine (5-bromo-2-deoxyuridine, BrdU) is a synthetic nucleoside which is an analogue of thymidine. BrdU is commonly used in the detection of proliferating cells in living tissues.

BrdU can be incorporated into the newly synthesized DNA of replicating cells (during the S phase of the cell cycle), substituting for thymidine during DNA replication. Antibodies specific for BrdU can then be used to detect the incorporated chemical (see immunohistochemistry), thus indicating cells that were actively replicating their DNA. Binding of the antibody requires denaturation of the DNA, usually by exposing the cells to acid or heat.

Because BrdU can replace thymidine during DNA replication, it can cause mutations, and its use is therefore potentially a health hazard.

ee also

* 5-Bromouracil

External links

* [http://www.wellesley.edu/Biology/Concepts/Html/brdu.html BrdU incorporation during DNA replication] - Jiayang Chien, Wellesley College


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Look at other dictionaries:

  • bromodeoxyuridine — ¦brōmō(ˌ)dē¦äksēˌ noun Etymology: brom + deoxy + uridine : a mutagenic analogue C9H11O5NBr of thymidine that induces chromosomal breakage especially in heterochromatic regions and has been used to selectively destroy actively dividing cells; 5… …   Useful english dictionary

  • bromodeoxyuridine — noun A synthetic nucleoside which is an analogue of thymidine; it is commonly used in the detection of proliferating cells in living tissues, where it competes with uridine …   Wiktionary

  • bromodeoxyuridine — A compound that competes with uridine for incorporation in RNA and fluoresces in ultraviolet light; used in BrDu banding. * * * bro·mo·de·oxy·ur·i·dine .brō mō .dē .äk sē yu̇r ə .dēn, dən or 5 bro·mo·de·oxy·ur·i·dine fīv n a mutagenic analog… …   Medical dictionary

  • bromodeoxyuridine — bro·mo·de·oxy·uridine …   English syllables

  • 5-bromodeoxyuridine — 5 bromodeoxyuridine. См. 5 бромдезоксиуридин. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • 5-bromodeoxyuridine — (BrdU) (BUdR) 5 bro·mo·de·oxy·uri·dine (BrdU) (BUdR) (bro″mo de ok se uґrĭ din) a thymidine analogue that can be incorporated into DNA during replication and is used as a mutagen in genetic research; it causes breakage in chromosomal… …   Medical dictionary

  • BDUR — bromodeoxyuridine …   Medical dictionary

  • BrdUrd — bromodeoxyuridine …   Medical dictionary

  • BDUR — • bromodeoxyuridine …   Dictionary of medical acronyms & abbreviations

  • BrdU — • bromodeoxyuridine …   Dictionary of medical acronyms & abbreviations

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