Acridine orange

Acridine orange

Chembox new
Name = Acridine orange
ImageFile = Acridine Orange.png ImageName = Acridine orange
IUPACName = N,N,N',N'-tetramethylacridine-
3,6-diamine
OtherNames = Euchrysine
3,6-Acridinediamine
Waxoline Orange A
Acridine Orange Base
Solvent Orange 15
Rhoduline Orange
Rhoduline Orange N
Acridine Orange NO
Rhoduline Orange NO
Basic Orange 14
Section1 = Chembox Identifiers
SMILES = CN(C)C1=CC2=C(C=C1)C=C3C=CC(=CC3=N2)N(C)C
CASNo = 494-38-2
PubChem=62344
RTECS = AR7601000
Section2 = Chembox Properties
C=17|H=19|N=3
Appearance = Orange powder
Section7 = Chembox Hazards
EUClass = Hazchem_Xi Hazchem_N
NFPA-H = 2
NFPA-F = 0
NFPA-R = 0
SPhrases = S26 S28 S37 S45

Acridine orange is a nucleic acid selective fluorescent cationic dye useful for cell cycle determination. It is cell-permeable, and interacts with DNA and RNA by intercalation or electrostatic attractions. When bound to DNA, it is very similar spectrally to fluorescein, with an excitation maximum at 502 nm and an emission maximum at 525 nm (green). When it associates with RNA, the excitation maximum shifts to 460 nm (blue) and the emission maximum shifts to 650 nm (red). Acridine orange will also enter acidic compartments such as lysosomes and become protonated and sequestered. In these low pH conditions, the dye will emit orange light when exited by blue light. The dye is often used in epifluorescence microscopy.

Acridine orange is prepared from coal tar and creosote oil.

Acridine orange can be used in conjunction with ethidium bromide to differentiate between live and apoptotic cells.

References


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Look at other dictionaries:

  • acridine orange — acridine orange. См. оранжевый акридин. (Источник: «Англо русский толковый словарь генетических терминов». Арефьев В.А., Лисовенко Л.А., Москва: Изд во ВНИРО, 1995 г.) …   Молекулярная биология и генетика. Толковый словарь.

  • acridine orange — 3,6 bis(dimethylamino)acridine hydrochloride; a basic fluorescent dye useful as a metachromatic stain for nucleic acid s; also used in screening cervical smears for abnormal and malignant cells, where unusual amounts of DNA and RNA occur during… …   Medical dictionary

  • acridine orange — A fluorescent vital dye, that intercalates into nucleic acids. The nuclei of stained cells fluoresce green; cytoplasmic RNA fluoresces orange. Acridine orange also stains acid mucopolysaccharides, and is widely used as a pH sensitive dye in… …   Dictionary of molecular biology

  • acridine orange — noun : an orange acridine dye see dye table I (under Basic Orange 14) …   Useful english dictionary

  • acridine orange — noun Date: circa 1909 a basic orange dye structurally related to acridine and used especially to stain nucleic acids …   New Collegiate Dictionary

  • acridine orange — noun A cationic dye, prepared from coal tar and creosote oil, often used in epifluorescence microscopy …   Wiktionary

  • Acridine orange — Акридиновый оранжевый (пигмент) …   Краткий толковый словарь по полиграфии

  • Acridine — Chembox new ImageFile=Acridine chemical structure.png ImageSize=250px IUPACName=Acridine OtherNames= Section1= Chembox Identifiers CASNo=260 94 6 PubChem=9215 SMILES=C1=CC=C2C(=C1)C=C3C=CC=CC3=N2 Section2= Chembox Properties C=13|H=9|N=1… …   Wikipedia

  • Acridine yellow — chembox new ImageFile = Acridine yellow.png IUPACName = 2,7 dimethylacridine 3,6 diamine OtherNames = Acridine yellow G Acridine yellow H107 Basic Yellow K 2,7 Dimethylproflavine Abbreviations = Formula = C15H15N3 MolarMass = 273.3g/mol CASNo =… …   Wikipedia

  • acridine — ● acridine nom féminin (latin acrido, inis, odeur piquante) Composé C13H9N, faiblement basique, base de la fabrication de colorants. (L acridine est utilisée comme mutagène expérimental en analyse génétique.) Composé qui dérive de l acridine par… …   Encyclopédie Universelle

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