3,3’,5,5’-Tetramethylbenzidine
- 3,3’,5,5’-Tetramethylbenzidine
Chembox new
Name = 3,3’,5,5’-Tetramethylbenzidine
ImageFile = 3,3’,5,5’-Tetramethylbenzidine.png ImageName = Line Structure
ImageFile1 = 3,3’,5,5’-Tetramethylbenzidine 3D-Ball-Stick.png ImageName1 = 3D Ball and Stick
ImageFile2 = 3,3’,5,5’-Tetramethylbenzidine Space-Filling.png ImageName2 = Space-filling
IUPACName = 3,3',5,5'-tetramethylbiphenyl-4,4'-diamine
Section1 = Chembox Identifiers
CASNo = 54827-17-7
SMILES = NC1=C(C)C=C(C2=CC(C)=C(N)C(C)=C2)C=C1C
Section2 = Chembox Properties
Formula = C16H20N2
MolarMass = 240.3482 g/mol
Density = ? g/cm3
MeltingPt = 192–196 °C
BoilingPt = °C
Section7 = Chembox Hazards
ExternalMSDS = [http://www.physchem.ox.ac.uk/MSDS/TE/3,3',5,5'-tetramethylbenzidine.html External MSDS]
3,3’,5,5’-Tetramethylbenzidine or TMB is a chromogenic substrate used in staining procedures in immunohistochemistry as well as being a visualising reagent used in enzyme-linked immunosorbent assays (ELISA) [ [http://www.sigmaaldrich.com/catalog/search/ProductDetail/ALDRICH/860336 860336 3 3 5 5 Tetramethylbenzidine ≥99% ] ] . TMB is a white crystal powder which forms a pale blue-green liquid in solution with ethyl acetate.
In solution, TMB forms a "blue" product when allowed to react with peroxidase enzymes such as horseradish peroxidase. The resulting colour change can be read on a spectrophotometer at a wavelength of 370 or 655 nm. Completion of the reaction results in a "yellow" coloration which may be read at 450 nm. The reaction can be halted by addition of acid or another stop reagent.
Material Safety
TMB should be kept out of direct sunlight as it is photosensitive. It should also be handled as a mutagen.
References
External links
* [http://biomeda.com/tetramethylbenzidine-tmb-ready-to-use-chromogen-100-ml.S18.html Tetramethylbenzidine (TMB) - Ready to Use Chromogen]
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