MOPS

MOPS

Chembox new
ImageFile=MOPS.pngImageSize=200px
IUPACName=3-morpholinopropane-1-sulfonic acid
OtherNames=3-(N-morpholino)propanesulfonic acid
Section1= Chembox Identifiers
CASNo=1132-61-2
PubChem=70807
SMILES=C1COCCN1CCCS(=O)(=O)O
Section2= Chembox Properties
Formula=C7H15NO4S
MolarMass=209.2633
Appearance=
Density=
MeltingPt=
BoilingPt=
Solubility=
Section3= Chembox Hazards
MainHazards=
FlashPt=
Autoignition=

MOPS is the common name for the compound 3-(N-morpholino)propanesulfonic acid a buffer introduced by Good et al. in the 1960's. It is a structural analog to MES. [Good, N.E. et al., Biochemistry, 5, 467-477 (1966).] Its chemical structure contains a morpholine ring. HEPES is a similar pH buffering compound which contains a piperazine ring. With a pKa of 7.20, MOPS is an excellent buffer for many biological systems at near-neutral pH.

Applications

MOPS is frequently used as a buffering agent in biology and biochemistry. It is has been tested and recommended for polyacrylamide gel electrophoresis. [Thomas, J.M. and Hodes, M.E., Analytical Biochemistry, 118, 194-196 (1981).] Usage above 20mM in mammalian cell culture work is not recommended. [Eagle, H., Science, 174, 500-503 (1971).]

References

See also

*MES
*HEPES
*CAPSO
*HEPPS

External links

* [http://www.jtbaker.com/msds/englishhtml/m8014.htm Safety information]
* [http://www2.sigmaaldrich.com/suite7/Area_of_Interest/Research_Essentials/Biological_Buffers/Key_Resources/BufferCalc/SigmaAldrich_Buffer_Calculator.html?cm_mmc=wiki-_-social-_-buffer-_-MOPS Sigma Aldrich Buffer Calculator] - Useful tool to calculate weight, volume, or concentration from molecular weight.


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