PIPES

Chembox new
Name = PIPES
ImageFile = PIPES_Molecule.jpg
ImageName = Chemical structure of PIPES
IUPACName =1,4-Piperazinediethanesulfonic acid (IUPAC)
OtherNames = PIPES
Section1 = Chembox Identifiers
CASNo = 5625-37-6
Section2 = Chembox Properties
Formula = C₈H₁₈N₂O₆S₂
MolarMass = 302.37
Appearance = White powder
Solubility = 1 g/L (100°C)
MeltingPt = Decomposes above 300°C
BoilingPt = Decomposes
Section7 = Chembox Hazards
ExternalMSDS = [http://www.jtbaker.com/msds/englishhtml/p4789.htm External MSDS]
MainHazards = Irritant
NFPA-H = 1
NFPA-R = 0 | Contact=2
NFPA-F = 0

PIPES is the common name for piperazine-N,N′-bis(2-ethanesulfonic acid), a frequently used buffering agent in biochemistry. It is an ethanesulfonic acid buffer developed by Good et al in the 1960s. [Good, N.E. et al., Biochemistry, 5, 456-477 (1966).]

Applications

PIPES has pKa near the physiological pH which makes it useful in cell culture work. PIPES has been documented minimizing lipid loss when buffering glutaraldehyde histology in plant and animal tissues. [Salema, R. and Brando, I., J. Submicr. Cytol., 9, 79 (1973).] [Schiff, R.I. and Gennaro, J.F., Scaning Electron Microsc., 3, 449 (1979).] Fungal zoospore fixation for fluorescence microscopy and electron microscopy were optimized with a combination of glutaraldehyde and formaldehyde in PIPES buffer. [Hardham, A.R., J. Histochem., 33, 110 (1985).] It has a negligible capacity to bind divalent ions.

References

External links

[http://www2.sigmaaldrich.com/suite7/Area_of_Interest/Research_Essentials/Biological_Buffers/Key_Resources/BufferCalc/SigmaAldrich_Buffer_Calculator.html?cm_mmc=wiki-_-social-_-buffer-_-PIPES Sigma Aldrich Buffer Calculator] - Useful tool to calculate weight, volume, or concentration from molecular weight.