- Insect diuretic hormones
Insect diuretic hormones are
hormone s that regulatewater balance throughdiuretic action.The
insect excretory system , responsible for regulating water balance in the insect, comprises theMalpighian tubules and thehindgut (theileum andrectum ). Malpighian tubules secrete primaryurine , most of which is passed into the hindgut where water,ion s and essential metabolites are reabsorbed before the fluid is excreted. Excretion is under the control ofdiuretic and anti-diuretic factors, orhormones , comprehensively reviewed by Coast et al. (2002).These factors are produced in
neurosecretory cell s in the insectnervous system , and stored and released from neurohaemal sites, such as thecorpora cardiaca in thebrain . Before a factor can be attributed with the role of hormone, it needs to meet certain criteria. While there is evidence that some diuretic and antidiuretic factors are indeed circulating neurohormones, this has not been demonstrated for all factors investigated so far.It has been known for many years that insects possess diuretic and antidiuretic factors, but it has only been comparatively recently that technological advances have allowed for them to be characterised. Antidiuretic factors are less well studied than diuretic factors. They act either on the Malpighian tubules to inhibit urine production, or on the hindgut to stimulate reabsorption. To date, the only insect for which both diuretic and antidiuretic hormones (acting directly on tubules) have been isolated is a beetle, the
mealworm "Tenebrio molitor" (Tenebrionidae).Functions of diuretic and antidiuretic hormones include: postprandial diuresis,
post-eclosion diuresis , excretion of excess metabolic water, clearance of toxic wastes and restrictingmetabolite loss (Coast et al., 2002).The effects of diuretic factors are tested in fluid secretion experiments, usually conducted using the
Ramsay assay , in which isolated Malpighian tubules are placed in droplets ofsaline solution under liquidparaffin . The ends of the tubules are drawn out of the saline and wrapped around minuten pins. The diameter of the secreted droplets is measured, whereby the volume, in nanolitres, and hence rate of secretion, can be calculated. Test substances are added to the saline and changes in secretion rate are recorded. Isolated tubules continue to secrete for many hours, and because the Ramsay assay is fairly easy to perform, many peptides are tested using this method. As a result, the effects of diuretic peptides are often tested only on the tubules and their role in other parts of the insect excretory system is not investigated.There are three main families of diuretic hormones: the
corticotropin-releasing factor (CRF)-related peptides,calcitonin (CT)-like peptides and the insectkinin s (Coast et al., 2002). These will be reviewed briefly. Others have been identified, but will not be discussed here.CRF-related peptides are the best characterised and have been isolated from "
Blattodea ",Isoptera ,Orthoptera ,Coleoptera ,Diptera andLepidoptera . Insect CRF-related peptides are so-called because of their similarity to the CRF-related peptides ofvertebrate s, which indicates a longevolution ary history. They range from 30 to 47amino acid residues in length. Although only a few orders are represented so far, CRF-related peptides are suspected to be ubiquitous in insects. They act by increasingcyclic AMP production in Malpighian tubules and appear to stimulatecation (K+/Na+) transport.Originally known as the myokinins because of their myotropic activity, the
kinin s were first isolated from the Madeira cockroach, Leucophaea maderae and the cricket, Acheta domesticus. Kinins are relatively smaller than the CRF-related peptides (typically 6 - 15 residues long). They appear to have a non-selective effect onsodium chloride andpotassium chloride secretion in tubules by opening a Ca2+-activated anion conductance, thus allowing more Cl- into the tubule. With the increase in available Cl-, additional Na+ and K+ can be transported into the lumen. Their effects are mimicked by the pharmacological agent, thapsigargin, which is sometimes used in fluid secretion assays when a kinin is not available. Kinins are known from Blattodea, Orthoptera, Lepidoptera and Diptera.Comparatively recently, a peptide was isolated from the cockroach
Diploptera punctata that showed no similarity to any known insect peptide but did show some similarity to vertebrate calcitonin (Furuya et al., 2000). The D. punctata peptide, subsequently named Dippu-DH31, turned out to be the first example of a whole new family of insect diuretic peptides – the calcitonin (CT)-like peptides. Dippu-DH31 was isolated using a cyclic AMP assay at the same time as a CRF-related peptide from the same insect. Ct-like peptides and CRF-related peptides both stimulate cAMP production by isolated tubules, but it is suspected that CT-like peptides target a different cAMP-dependent effector system or activate a different second messenger pathway. CT-like peptides have since been identified in other orders – Diptera, Lepidoptera, and one has been partially sequenced from Hymenoptera. Unpublished studies show immunologically-related peptides in Tenebrio molitor (Coast et al., 2002).It appears that all insects possess diuretic factors from two or more families, indicating that fluid balance is very finely controlled. Synergism between the different factors has been demonstrated in a number of
species . However, although it is common, it should not be assumed to be universal. Also, in many cases, it is only the effects on fluid secretion that are measured, and while two factors may both increase fluid secretion, their effects on ion transport may be very different. Further studies examining the composition of the secreted fluid and electrophysiological experiments that explore ion movement, will shed more light on the actual physiological function of these factorsin vivo .For example, in vivo experiments have demonstrated that the fluid secreted by the Malpighian tubules of the desert beetle, Onymacris plana is directed to the midgut for recycling to the
haemolymph . In this way, metabolic wastes are rapidly cleared from the haemolymph without an associated loss of water, indicating that diuretic hormones may not always effect diuresis per se (Nicolson, 1991).The rectal or cryptonephric complex is a structure in which the terminal parts of the Malpighian tubules are closely associated with the rectum. It withdraws water from the rectal contents, thereby limiting fecal water loss, and is even capable of withdrawing water from unsaturated air. The complex is only present in lepidopteran larvae and some beetle species. Little is known about the hormonal control of fluid uptake by the cryptonephric complex, which is certain to play an important role in the species in which it occurs.
Nomenclature
First three letters of
genus added to first two letters of the species name. For example, a 37-residue diuretic peptide from Tenebrio molitor will be named Tenmo-DH37.References
* Coast G.M., Orchard, I., Phillips, J.E. and Schooley, D.A. 2002. Insect Diuretic and Antidiuretic Hormones. Advances in Insect Physiology 29: 279-409.
* Furuya, K., Milchak., R.J., Schegg, K.M., Zhang, J., Tobe, S.S., Coast, G.M., Schooley, D.A., 2000. Cockroach diuretic hormones: characterization of a calcitonin-like peptide in insects. Proceedings of the National Academy of Sciences of the USA 97: 6469-6474.
* Nicolson, S.W. 1991. Diuresis or clearance: is there a physiological role for the diuretic hormone of the desert beetle "Onymacris". Journal of Insect Physiology 37: 447-452.
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