Glucose 6-phosphatase

Glucose 6-phosphatase (Glc-6-Pase) is an enzyme that hydrolyzes glucose-6-phosphate resulting in the creation of a phosphate group and free glucose. Glucose is then exported from the cell via glucose transporter membrane proteins.cite journal |author=Ghosh A, Shieh JJ, Pan CJ, Sun MS, Chou JY |title=The catalytic center of glucose-6-phosphatase. HIS176 is the nucleophile forming the phosphohistidine-enzyme intermediate during catalysis |journal=J. Biol. Chem. |volume=277 |issue=36 |pages=32837–42 |year=2002 |month=September |pmid=12093795 |doi=10.1074/jbc.M201853200 |url=] This catalysis completes the final step in gluconeogenesis and glycogenolysis and therefore plays a key role in the homeostatic regulation of blood glucose levels.cite book |author=Nordlie R et al. |title=The Enzymes of biological membranes, 2nd edition|publisher=Plenum Press |location=New York |year=1985 |pages=349-398 |isbn=0-306-41453-8 |oclc= |doi= |accessdate=]

The Glc-6-Pase family includes two functional phosphohydrolases; Glc-6-Pase-α and Glc-6-Pase-β, the former of which is the prototype. Glc-6-Pase-α and Glc-6-Pase-β share similar active site structure, topology, mechanism of action, and kinetic properties with respect to Glc-6-P hydrolysis.cite journal |author=Ghosh A, Shieh JJ, Pan CJ, Chou JY |title=Histidine 167 is the phosphate acceptor in glucose-6-phosphatase-beta forming a phosphohistidine enzyme intermediate during catalysis |journal=J. Biol. Chem. |volume=279 |issue=13 |pages=12479–83 |year=2004 |month=March |pmid=14718531 |doi=10.1074/jbc.M313271200 |url=]

tructure and Function

Although a clear consensus has not been reached, a large number of scientists adhere to a substrate-transport model to account for the catalytic properties of glucose-6-Pase. In this model, glucose 6-Pase has a low degree of selectivity. The transfer of the glucose 6 phosphate is carried out by a transporter protein (T1) and the endoplasmic reticulum (ER) contains structures allowing the exit of the phosphate group (T2) and glucose (T3).cite journal |author=van Schaftingen E, Gerin I |title=The glucose-6-phosphatase system |journal=Biochem. J. |volume=362 |issue=Pt 3 |pages=513–32 |year=2002 |month=March |pmid=11879177 |doi= |url=http://www.biochemj.org/bj/362/0513/bj3620513.htm]

Glucose-6-Pase consists of 357 amino acids, and is anchored to the endoplasmic reticulum (ER) by nine transmembrane helicies. Its N-terminal and active site are found on the lumen side of the ER and its C-terminus projects into the cytoplasm. Due to its tight association to the ER, the exact structure of glucose-6-Pase remains unknown. However, sequence alignment has shown that glucose-6-Pase is structurally similar to the active site of the vanadium-containing chloroperoxidase found in Curvularia inaequalis.cite journal |author=Pan CJ, Lei KJ, Annabi B, Hemrika W, Chou JY |title=Transmembrane topology of glucose-6-phosphatase |journal=J. Biol. Chem. |volume=273 |issue=11 |pages=6144–8 |year=1998 |month=March |pmid=9497333 |doi= |url=http://www.jbc.org/cgi/pmidlookup?view=long&pmid=9497333]

Based on pH kinetic studies of Glc-6-Pase-α catalysis, it was proposed that the hydrolysis of Glucose-6-Phosphate was completed via a covalent phosphohistidine glucose-6-Phosphate intermediate. The active site of Glc-6-Pase-α was initially identified by the presence of a conserved phosphate signature motif usually found in lipid phosphatases, acid phosphatases, and vanadium haloperoxidases.cite journal |author=Ghosh A, Shieh JJ, Pan CJ, Chou JY |title=Histidine 167 is the phosphate acceptor in glucose-6-phosphatase-beta forming a phosphohistidine enzyme intermediate during catalysis |journal=J. Biol. Chem. |volume=279 |issue=13 |pages=12479–83 |year=2004 |month=March |pmid=14718531 |doi=10.1074/jbc.M313271200 |url=]

Essential residues in the active site of vanadium haloperoxidases include: Lys353, Arg360, Arg490, His404, and His496. Corresponding residues in the active site of Glc-6-Pase-α include Arg170 and Arg83, which donate hydrogen ions to the phosphate to stabilize the transition state, His119, which provides a proton to the dephosphorylated oxygen attached to glucose, and His176, which completes a nucleophilic attack on the phosphate to form a covalently bound phosphoryl enzyme intermediate.cite journal |author=Ghosh A, Shieh JJ, Pan CJ, Sun MS, Chou JY |title=The catalytic center of glucose-6-phosphatase. HIS176 is the nucleophile forming the phosphohistidine-enzyme intermediate during catalysis |journal=J. Biol. Chem. |volume=277 |issue=36 |pages=32837–42 |year=2002 |month=September |pmid=12093795 |doi=10.1074/jbc.M201853200 |url=] Within the Vanadium-containing chloroperoxidase, Lys353 was found to stabilize the phosphate in the transition state. However, the corresponding residue in Glc-6-Pase-α (Lys76) resides within the ER membrane and its function, if any, is currently undetermined. With the exception of Lys76, these residues are all located on the luminal side of the ER membrane.cite journal |author=Ghosh A, Shieh JJ, Pan CJ, Chou JY |title=Histidine 167 is the phosphate acceptor in glucose-6-phosphatase-beta forming a phosphohistidine enzyme intermediate during catalysis |journal=J. Biol. Chem. |volume=279 |issue=13 |pages=12479–83 |year=2004 |month=March |pmid=14718531 |doi=10.1074/jbc.M313271200 |url=]

Glc-6-Pase-β is a ubiquitously expressed, 346-amino acid membrane protein that shares 36% sequence identity with Glc-6-Pase-α. Within the Glc-6-Pase-β enzyme, sequence alignments predict that its active site contains His167, His114, and Arg79. Similar to that of the Glc-6-Pase-α active site, His167 is the residue that provides the nucleophilic attack, and His114, and Arg79 are the hydrogen donors. Glc-6-Pase-β is also localized in the ER membrane, although its orientation is unknown.cite journal |author=Ghosh A, Shieh JJ, Pan CJ, Chou JY |title=Histidine 167 is the phosphate acceptor in glucose-6-phosphatase-beta forming a phosphohistidine enzyme intermediate during catalysis |journal=J. Biol. Chem. |volume=279 |issue=13 |pages=12479–83 |year=2004 |month=March |pmid=14718531 |doi=10.1074/jbc.M313271200 |url=]

Mechanism

The hydrolysis of the Glc-6-P begins with a nucleophilic attack on the sugar-bound phosphate by His176 resulting in the formation of a phosphohistidine bond and the degredation of a carbonyl. A Negatively charged oxygen then transfers its electrons reforming a carbonyl and breaking its bond with glucose. The negatively charged glucose-bound oxygen is then protonated by His119 forming a free glucose. The phospho-intermediate produced by the reaction between His176 and the phosphate group is then broken by a hydrophilic attack; after the addition of another hydroxide and the decomposition of a carbonyl, the carbonyl is reformed kicking off the electrons originally donated by the His176 residue thereby creating a free phosphate group and completing the hydrolysis.cite journal |author=Ghosh A, Shieh JJ, Pan CJ, Sun MS, Chou JY |title=The catalytic center of glucose-6-phosphatase. HIS176 is the nucleophile forming the phosphohistidine-enzyme intermediate during catalysis |journal=J. Biol. Chem. |volume=277 |issue=36 |pages=32837–42 |year=2002 |month=September |pmid=12093795 |doi=10.1074/jbc.M201853200 |url=]

Expression

Genes coding for the enzyme are primarily expressed in the liver and kidney cortex and to a lesser extent the b-cells of the pancreatic islets and intestinal mucosa (esp. during times of starvation.cite journal |author=van Schaftingen E, Gerin I |title=The glucose-6-phosphatase system |journal=Biochem. J. |volume=362 |issue=Pt 3 |pages=513–32 |year=2002 |month=March |pmid=11879177 |doi= |url=http://www.biochemj.org/bj/362/0513/bj3620513.htm] According to Surholt and Newsholme, Glc 6-Pase is present in a wide variety of muscles across the animal kingdom, albeit at very low concentrations. [1] . The enzyme plays and important role during periods of fasting and when glucose levels are low. It has been shown that starvation and diabetes induces a 2-3-fold increase in Glc-6-Pase activity in the liver.cite journal |author=van Schaftingen E, Gerin I |title=The glucose-6-phosphatase system |journal=Biochem. J. |volume=362 |issue=Pt 3 |pages=513–32 |year=2002 |month=March |pmid=11879177 |doi= |url=http://www.biochemj.org/bj/362/0513/bj3620513.htm] Glc 6-Pase activity also increases dramatically at birth when an organism becomes independent of the mothers source of glucose. The human Glc 6-Pase gene contains five exons spanning approximately 125.5 kb DNA located on chromosome 17q21.cite journal |author=Angaroni CJ, de Kremer RD, Argaraña CE, "et al" |title=Glycogen storage disease type Ia in Argentina: two novel glucose-6-phosphatase mutations affecting protein stability |journal=Mol. Genet. Metab. |volume=83 |issue=3 |pages=276–9 |year=2004 |month=November |pmid=15542400 |doi=10.1016/j.ymgme.2004.06.010 |url= |issn=]

Clinical Significance

Mutations in Glc-6-Pase-α can lead to the glycogen storage disease type 1a called von Gierke's disease, which is characterized by loss of blood glucose homeostasis and disorders of glycogen and lipid metabolism. cite book |author=Nordlie R et al. |title=The Enzymes of biological membranes, 2nd edition|publisher=Plenum Press |location=New York |year=1985 |pages=349-398 |isbn=0-306-41453-8 |oclc= |doi= |accessdate=]

ee also

* Hexokinase

Notes

Molecular graphics images were produced using UCSF Chimera.cite journal |author=Pettersen EF, Goddard TD, Huang CC, "et al" |title=UCSF Chimera--a visualization system for exploratory research and analysis |journal=J Comput Chem |volume=25 |issue=13 |pages=1605–12 |year=2004 |month=October |pmid=15264254 |doi=10.1002/jcc.20084 |url=]

References

External links

*
*, Gene|G6PC2, Gene|G6PC3, Gene|G6PR
*
*