Flavoprotein

Flavoprotein

Flavoproteins are proteins that contain a nucleic acid derivative of riboflavin: the flavin adenine dinucleotide (FAD) or flavin mononucleotide (FMN).

Flavoproteins are involved in a wide array of biological processes, including, but by no means limited to, bioluminescence, removal of radicals contributing to oxidative stress, photosynthesis, DNA repair, and apoptosis. The spectroscopic properties of the flavin cofactor make it a natural reporter for changes occurring within the active site; this makes flavoproteins one of the most-studied enzyme families.

Discovery

The first mention of a flavoprotein in the scientific literature dates back to 1879, when the work on the composition of cow’s milk resulted in the isolation of a bright-yellow pigment, that we now know as flavin, but termed lactochrome at the time. By the early 1930s, this same pigment had been isolated from a range of sources, and recognised as a component of the vitamin B complex. Its structure was determined almost simultaneously by two groups in 1934, and given the name riboflavin, derived from the ribityl side chain and yellow colour of the conjugated ring system.Massey, V. (2000) The chemical and biological versatility of riboflavin, Biochem. Soc. Trans. 28, 283-296.]

The first evidence for the requirement of flavin as an enzyme cofactor came in 1935. Hugo Theorell and coworkers showed that a bright-yellow-coloured yeast protein, identified previously as essential for cellular respiration, could be separated into apoprotein and a bright-yellow pigment. Neither apoprotein nor pigment alone could catalyse the oxidation of NADH, but mixing of the two restored the enzyme activity. However, replacing the isolated pigment with riboflavin did not restore enzyme activity, despite their being indistinguishable under spectroscopy. This led to the discovery that the protein studied required not riboflavin but flavin mononucleotide to be catalytically active ] [Theorell, H. (1935) Preparation in pure state of the effect group of yellow enzymes, Biochemische Zeitschrift 275, 344-346.]

Similar experiments with D-amino acid oxidase [Warburg, O., and Christian, W. (1938) Isolation of the prosthetic group of the amino acid oxydase, Biochemische Zeitschrift 298, 150-168.] led to the identification of flavin adenine dinucleotide (FAD) as a second form of flavin utilised by enzymes. [Christie, S. M. H., Kenner, G. W., and Todd, A. R. (1954) NUCLEOTIDES .25. A SYNTHESIS OF FLAVIN ADENINE DINUCLEOTIDE, Journal of the Chemical Society, 46-52.]

ee also

Phototropin
Cryptochrome

References

External links

*
* The menu "science" of the program [http://3d-alignment.eu/ STRAP] provides A comprehensive collection of all flavo-proteins with known 3D-structure. It compares the protein structures to elucidate phylogenetic relationships.


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Look at other dictionaries:

  • flavoprotein — [flā΄vō prō′tēn΄] n. any of a group of enzymes, involved in tissue respiration, consisting of a protein and a flavin group …   English World dictionary

  • flavoprotein — flavoproteinas statusas T sritis chemija apibrėžtis Baltymas, turintis flavininį nukleotidą. atitikmenys: angl. flavoprotein rus. флавопротеин …   Chemijos terminų aiškinamasis žodynas

  • flavoprotein — noun Etymology: International Scientific Vocabulary flavin + o + protein Date: 1934 a dehydrogenase that contains a flavin and often a metal and plays a major role in biological oxidations …   New Collegiate Dictionary

  • Flavoprotein — Flavoproteine sind Proteine, die als Kofaktor Flavinnukleotide (Flavinadenindinukleotid (FAD), Flavinmononukleotid (FMN)) enthalten. Es handelt sich um Enzyme, die Redox Reaktionen katalysieren, wobei Flavin als Elektronenüberträger dient, der… …   Deutsch Wikipedia

  • flavoprotein — /flay voh proh teen, tee euhn/, n. Biochem. an enzyme, containing riboflavin and linked chemically with a protein, active in the oxidation of foods in animal cells. [1930 35; FLAVO + PROTEIN] * * * …   Universalium

  • flavoprotein — noun any of a group of enzymes, containing flavin, that act as dehydrogenases …   Wiktionary

  • flavoprotein — A compound protein possessing a flavin as prosthetic group. Cf.:flavoenzyme. * * * fla·vo·pro·tein .flāv ō prō .tēn, .flav , prōt ē ən n a dehydrogenase that contains a flavin and often a metal and plays a major role in biological oxidations * *… …   Medical dictionary

  • Flavoprotein — Fla|vo|pro|te|in [↑ Flavin u. ↑ Protein] svw. ↑ Flavoenzym …   Universal-Lexikon

  • flavoprotein — n. enzyme that aids in the oxidization of food …   English contemporary dictionary

  • flavoprotein — fla·vo·protein …   English syllables

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