3,5-Dinitrosalicylic acid
- 3,5-Dinitrosalicylic acid
3,5-Dinitrosalicylic acid (DNS) is an aromatic compound that reacts with reducing sugars and other reducing molecules to form 3-amino-5-nitrosalicylic acid, which absorbs light strongly at 540 nm. It was first introduced as a method to detect reducing substances in urine and has since been widely used, for example, for quantificating carbohydrates levels in blood. It is mainly used in assay of alpha-amylase. However, enzymatic methods are usually preferred to DNS due to their specificity.
External links
* [http://www.glue.umd.edu/~NSW/ench485/lab4a.htm Description of lab use from the "Department of Chemical Engineering, University of Maryland"]
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